MilliporeSigma
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918741

Sigma-Aldrich

TissueFab® bioink 

(Gel)ma -VIS/405nm, low endotoxin

Synonym(s):
Bioink, GelMA, Gelatin methacrylamide, Gelatin methacrylate, Gelatin methacryloyl

Quality Level

description

suitable for 3D bioprinting applications
with LAP photoinitiator

sterility

sterile-filtered

form

viscous liquid

impurities

≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
<50 EU/mL Endotoxin

color

colorless to pale yellow

particle size

0.2 μm

pH

6.5-7.5

application(s)

3D bioprinting

storage temp.

2-8°C

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sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered (0.2μm)

form

viscous liquid

form

-

form

viscous liquid (gel)

form

viscous liquid (gel)

impurities

≤5 CFU/g Bioburden (Fungal)

impurities

-

impurities

<5 CFU/g Bioburden (Fungal), <5 CFU/g Bioburden (Total Aerobic)

impurities

<5 CFU/g Bioburden

color

colorless to pale yellow

color

-

color

colorless to pale yellow

color

pale yellow to colorless

particle size

0.2 μm

particle size

-

particle size

0.2 μm

particle size

-

Application

TissueFab® GelMA-Vis-LAP bioink is a gelatin methacryloyl (GelMA) based bioink for 3D bioprinting applications. LAP is used as the photoinitiator, which allows blue light photocrosslinking of the printed structure. The formulation is optimized for high printing fidelity and cell viability. The low endotoxin level of this product is lower than 50 EU/mL.

Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation.

Legal Information

TISSUEFAB is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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25G
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MMYOMAG-74K-13

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Y Shi et al.
Biomedical materials (Bristol, England), 13(3), 035008-035008 (2018-01-09)
Three-dimensional bioprinting is an emerging technology for fabricating living 3D constructs, and it has shown great promise in tissue engineering. Bioinks are scaffold materials mixed with cells used by 3D bioprinting to form a required cell-laden structure. In this paper
Wanjun Liu et al.
Advanced healthcare materials, 6(12) (2017-05-04)
Bioprinting is an emerging technique for the fabrication of 3D cell-laden constructs. However, the progress for generating a 3D complex physiological microenvironment has been hampered by a lack of advanced cell-responsive bioinks that enable bioprinting with high structural fidelity, particularly
B Duan et al.
Acta biomaterialia, 10(5), 1836-1846 (2013-12-18)
Tissue engineering has great potential to provide a functional de novo living valve replacement, capable of integration with host tissue and growth. Among various valve conduit fabrication techniques, three-dimensional (3-D) bioprinting enables deposition of cells and hydrogels into 3-D constructs
Weitao Jia et al.
Biomaterials, 106, 58-68 (2016-08-24)
Despite the significant technological advancement in tissue engineering, challenges still exist towards the development of complex and fully functional tissue constructs that mimic their natural counterparts. To address these challenges, bioprinting has emerged as an enabling technology to create highly
Wanjun Liu et al.
Biofabrication, 10(2), 024102-024102 (2017-11-28)
Bioinks with shear-thinning/rapid solidification properties and strong mechanics are usually needed for the bioprinting of three-dimensional (3D) cell-laden constructs. As such, it remains challenging to generate soft constructs from bioinks at low concentrations that are favorable for cellular activities. Herein

Articles

3D Bioprinting: Bioink Selection Guide

Bioinks can be 3D bioprinted into functional tissue constructs for drug screening, disease modeling, and in vitro transplantation. Choose the Bioinks and method for specific tissues engineering applications.

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