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Sigma-Aldrich

TissueFab® bioink 

Crosslinking solution, low endotoxin

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Synonym(s):
Calcium chloride solution

Quality Level

description

suitable for 3D bioprinting applications

sterility

sterile-filtered

form

viscous liquid

impurities

≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
<25 EU/mL Endotoxin

color

colorless to light yellow

particle size

0.1 μm

pH

6.5-7.5

application(s)

3D bioprinting

storage temp.

2-8°C

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Application

TissueFab® - crosslinking solution is 200 mM calcium chloride solution, it is a crosslinking agent for chemical crosslinking of bioinks that contain alginate. Alginate is a naturally occurring anionic polymer typically obtained from brown seaweed, and has been extensively in bioink formulations, due to its biocompatibility, structural similarity with extracellular matrix of living tissues, and mild gelation. The sodium salt of alginic acid can be crosslinked by exposing to the crosslinker solution containing divalent Ca2+ cation.

Packaging

5 x 10ml case pack

Legal Information

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Wee et al.
Advanced drug delivery reviews, 31(3), 267-285 (2000-06-06)
There are a variety of both natural and synthetic polymeric systems that have been investigated for the controlled release of proteins. Many of the procedures employed to incorporate proteins into a polymeric matrix can be harsh and often cause denaturation
Kuen Yong Lee et al.
Progress in polymer science, 37(1), 106-126 (2011-11-30)
Alginate is a biomaterial that has found numerous applications in biomedical science and engineering due to its favorable properties, including biocompatibility and ease of gelation. Alginate hydrogels have been particularly attractive in wound healing, drug delivery, and tissue engineering applications

Articles

Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.

Learn how 3D bioprinting is revolutionizing drug discovery with highly-controllable cell co-culture, printable biomaterials, and its potential to simulate tissues and organs. This review paper also compares 3D bioprinting to other advanced biomimetic techniques such as organoids and organ chips.

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