MilliporeSigma
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920975

Sigma-Aldrich

TissueFab® bioink 

(GelAlgHA)ma -UV/365 nm

Synonym(s):
AlgMA, Alginate, Bioink, GelMA, Gelatin, HAMA, Hyaluronic acid

Quality Level

description

suitable for 3D bioprinting applications

sterility

sterile-filtered

form

viscous liquid (or gel)

impurities

<5 CFU/g Bioburden (Fungal)
<5 CFU/g Bioburden (Total Aerobic)

color

colorless to pale yellow

particle size

0.2 μm

pH

(6.5-7.5)

application(s)

3D bioprinting

storage temp.

2-8°C

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description

suitable for 3D bioprinting applications

description

suitable for 3D bioprinting applications

description

0.2 μm sterile filtered, suitable for 3D bioprinting applications

description

-

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

0.2 μm filtered

form

viscous liquid (or gel)

form

viscous liquid

form

viscous liquid

form

viscous liquid ((or gel))

impurities

<5 CFU/g Bioburden (Fungal)

impurities

≤5 CFU/g Bioburden (Fungal), ≤5 CFU/g Bioburden (Total Aerobic), <25 EU/mL Endotoxin

impurities

≤5 CFU/g Bioburden (Fungal), ≤5 CFU/g Bioburden (Total Aerobic)

impurities

<5 CFU/g Bioburden (Total Aerobic), <5 CFU/g Bioburden (fungal), <50 EU/mL Endotoxin

color

colorless to pale yellow

color

colorless to light yellow

color

colorless to pale yellow

color

colorless to pale yellow

particle size

0.2 μm

particle size

0.1 μm

particle size

-

particle size

-

Application

TissueFab®- HAMA-AlgMA-UV bioink is an alginate methacrylate based bioink for 3D bioprinting applications. The formulation is optimized for 3D bioprinting of tissues and constructs using extrusion based 3D bioprinters, and can be can be used to bioprint cell-laden hydrogels in desired shape without any supporting material. The crosslinking of printed structures can be done in one step using UV light for further culture and maturation of cells for tissue engineering and regenerative medicine applications.

This bioink is derived from natural polymers − hyaluronic acid, alginate and gelatin. Hyaluronic acid is also known as sodium hyaluronate or hyaluronan, is an anionic, nonsulfated glycosaminoglycan (GAG). Alginate also known as sodium alginate or alginic acid, is a natural biopolymer obtained from brown algae. Alginate is a natural polysaccharide widely used in tissue engineering. Gelatin contains bioactive peptide sequences similar to native extracellular matrix which promotes integrin mediated cell adhesion and MMP sensitive enzymatic degradation which are essential for cellular functions such as migration, proliferation and differentiation.

Features and Benefits

  • Ready-to-use formulation optimized for high printing fidelity and cell viability, eliminating the lengthy bioink formulation development process
  • Step-by-step protocols developed and tested by MilliporeSigma 3D Bioprinting Scientists, no prior 3D bioprinting experience needed
  • Suitable for different extrusion-based 3D bioprinter model

Packaging

10 mL in glass bottle

Legal Information

TISSUEFAB is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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