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700001P

Avanti

DC-Cholesterol HCl

Avanti Polar Lipids 700001P, powder

Synonym(s):

3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C32H57N2O2Cl
CAS Number:
Molecular Weight:
537.26
UNSPSC Code:
12352211
NACRES:
NA.25

assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 g (700001P-1g)
pkg of 1 × 200 mg (700001P-200mg)
pkg of 1 × 25 mg (700001P-25mg)
pkg of 1 × 500 mg (700001P-500mg)

manufacturer/tradename

Avanti Polar Lipids 700001P

application(s)

vaccine development

shipped in

dry ice

storage temp.

−20°C

SMILES string

[H][C@](C1)(OC(NCC[N+](C)([H])C)=O)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@@H](C)CCCC(C)C.[Cl-]

InChI

1S/C32H56N2O2.ClH/c1-22(2)9-8-10-23(3)27-13-14-28-26-12-11-24-21-25(36-30(35)33-19-20-34(6)7)15-17-31(24,4)29(26)16-18-32(27,28)5;/h11,22-23,25-29H,8-10,12-21H2,1-7H3,(H,33,35);1H

InChI key

ISXSJGHXHUZXNF-UHFFFAOYSA-N

General description

DC-Cholesterol HCl is a cholesterol derivative and a cationic lipid with a head group and linker moiety.
DC-Cholesterol HCl is a sterol-based transfection reagent.

Application

DC-Cholesterol HCl is suitable:
  • for the preparation of ultrasound cationic microbubbles
  • to prepare liposomes
  • for the preparation of L-glutathione (GSH), loaded proliposomes

DC-Cholesterol HCl may be used in the preparation of colloidal dispersions for transmission electron microscopy studies. It may also be used in liposome preparation for assessing hexokinase activity, poly (lactic-coglycolicacid) loading and in mRNA transfection studies.

Biochem/physiol Actions

DC-Cholesterol is an effective transfection agent and a liposome reagent. Liposome of DC-Cholesterol with dioleoylphosphatidylethanolamine (DOPE) are efficient for DNA transfection. It imparts positive surface charge to the microbubbles for ultra sound based transfection of clustered regularly interspaced short palindromic repeats (CRISPRs).
DC-cholesterol is a cationic lipid, known to be the most efficient in gene transfection study.

Packaging

20 mL Clear Glass Screw Cap Vial (700001P-1g)
20 mL Clear Glass Screw Cap Vial (700001P-500mg)
5 mL Amber Glass Screw Cap Vial (700001P-200mg)
5 mL Amber Glass Screw Cap Vial (700001P-25mg)

also commonly purchased with this product

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pharmaceutical Chemistry ? I, 142-142 (2003)
Cationic nanoliposomes meet mRNA: efficient delivery of modified mRNA using hemocompatible and stable vectors for therapeutic applications
Michel T, et al.
Molecular Therapy, 8, 459-468 (2017)
C Colonna et al.
International journal of pharmaceutics, 364(1), 108-118 (2008-09-09)
The aim of this work was to investigate lipid-based dried powders as transfection competent carriers capable of promoting the expression of therapeutic genes. The lipid-based vectors were prepared by combining different cationic lipids 1,2-dioleoyl-3-trimethylammoniumpropane chloride (DOTAP), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)
Modulation of yeast hexokinase on bio-inspired membranes
Umakoshi H and Nishida A
Biochemical Engineering Journal, 69(5), 138-143 (2012)
Cabazitaxel-Loaded Nanocarriers for Cancer Therapy with Reduced Side Effects
Kommineni N, et al.
Pharmaceutics, 11(3) (2019)

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