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H-922

Supelco

α-Hydroxymidazolam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

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About This Item

Empirical Formula (Hill Notation):
C18H13ClFN3O
CAS Number:
Molecular Weight:
341.77
UNSPSC Code:
41116107
NACRES:
NA.24

grade

certified reference material

Quality Level

form

liquid

feature

Snap-N-Spike®/Snap-N-Shoot®

packaging

ampule of 1 mL

manufacturer/tradename

Cerilliant®

concentration

1.0 mg/mL in methanol

technique(s)

gas chromatography (GC): suitable
liquid chromatography (LC): suitable

application(s)

clinical testing

format

single component solution

storage temp.

2-8°C

SMILES string

OCc1ncc2CN=C(c3ccccc3F)c4cc(Cl)ccc4-n12

InChI

1S/C18H13ClFN3O/c19-11-5-6-16-14(7-11)18(13-3-1-2-4-15(13)20)22-9-12-8-21-17(10-24)23(12)16/h1-8,24H,9-10H2

InChI key

QHSMEGADRFZVNE-UHFFFAOYSA-N

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General description

α-Hydroxymidazolam is an active, major metabolite in urine and blood of the benzodiazepine midazolam. Midazolam is sold as Dormicum, Hypnovel®, and Versed as a sedative and treatment for insomnia and seizures. This Certified Spiking Solution® is suitable as starting material for use in calibrators or controls for a variety of LC/MS or GC/MS applications from forensic analysis and clinical toxicology to urine drug testing.

Application


  • Pharmacodynamic studies: α-Hydroxymidazolam is utilized in pharmacodynamic research to compare the effects of midazolam and its metabolites on neuronal activity. The study provides insight into the depressant effects on the neocortical neuronal network, aiding in the understanding of benzodiazepine drug actions (Balk et al., 2017).

  • Metabolic pathway research: Research explores the use of α-Hydroxymidazolam in studying drug-drug interactions and metabolism in cytochrome P450 knockout mice. This application highlights its role in examining metabolic pathways and interactions that affect drug metabolism, crucial for drug development and therapy optimization (Grimsley et al., 2013).

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Hypnovel is a registered trademark of Hoffman-LaRoche & Co. AG
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

target_organs

Eyes

wgk_germany

WGK 2

flash_point_f

closed cup

flash_point_c

closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xinping Xue et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 187-193 (2011-02-15)
In order to simultaneously determine in vivo P-glycoprotein (P-gp) and Cytochrome P450 3A (CYP3A) activity, a new, rapid and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed and fully validated to simultaneously determine midazolam (MDZ, as CYP3A substrate)
Camilla Svanström et al.
Journal of pharmaceutical and biomedical analysis, 58, 71-77 (2011-10-14)
The metabolic conversion of midazolam (MDZ) to its main metabolite 1'-hydroxy-midazolam (1-OH-MDZ) can be used as a probe drug for cytochrome P450 3A (CYP3A) activity. A sensitive method for the simultaneous determination of MDZ and its metabolite 1-OH-MDZ in human
Ruut Kaartama et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(19), 1668-1676 (2011-05-03)
A sensitive and selective gas chromatographic mass spectrometric method for the determination of midazolam and its biologically active metabolite, 1-hydroxymidazolam, in rabbit plasma has been developed and validated. Sample preparation includes mixed-mode solid-phase extraction and derivatization with silylating reagents. Midazolam-d4
High-throughput analysis of in vitro cytochrome p450 inhibition samples using mass spectrometry coupled with an integrated liquid chromatography/autosampler system.
Ann Brown et al.
Rapid communications in mass spectrometry : RCM, 24(8), 1207-1210 (2010-03-20)
Cyrille Marvalin et al.
Xenobiotica; the fate of foreign compounds in biological systems, 42(3), 285-293 (2011-10-26)
Midazolam, a potent benzodiazepine derivative and a typical substrate of CYP3A4/3A5, is essentially metabolized in human into 1'-hydroxymidazolam, then eliminated as the corresponding phase II metabolite, the 1'-O-β-D-glucuronide derivative. A high yield alternative to the current multistep synthesis of 1'-hydroxymidazolam

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