03-0190-00

SMC^® Human IL-18 High Sensitivity Immunoassay Kit

• Synonym(s): IGIF immunoassay kit, IL-18 Single Molecule Counting Kit, IL-18 ultra-sensitive immunoassay, Interleukin-18 immunoassay kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• species reactivity: mouse, feline, rat
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.09 pg/mL; (Lower Limit of Quantification); inter-assay cv: 7%; intra-assay cv: 4%; (five normal plasma samples run in triplicate by 3 different operators on 3 different days.)
• technique(s): single molecule counting: suitable
• storage temp.: 2-8°C

Description

General description

The SMC^® Human IL-18 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Specificity

Species Reactivity:
Specificity to the following species recombinant proteins were tested with the following results:
Feline – 0.5% Mouse – 4% Rat – 0.3%

Cross Reactivity:
No cross-reactivity was seen with the following cytokines: IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E, IL-7F, IL-22, IL-27.

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 99% with a range of 92-107%
• Minimum of 5 plasma samples spiked with average recovery of 95% with a range of 88-103%

Application

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human IL-18 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-18 in human serum and plasma.

This new streamline kit has the following features including:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Research Category:
Inflammation & Immunology

Research Sub-Category:

• Inflammatory Disease,
• Immune Response
• Autoimmune disease

Packaging

96 well assay

Components

• Assay Buffer
• IL-18 Coated Beads
• Standard Diluent
• IL-18 Detection Antibody
• IL-18 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects