03-185

Histone H2B human recombinant

• UNSPSC Code: 12352203
• eCl@ss: 32160405
• NACRES: NA.84

Properties

• antibody form: purified antibody
• Quality Level: 100
• assay: >50% (SDS-PAGE)
• technique(s): activity assay: suitable; western blot: suitable

Description

General description

Histone H2B is one of four core histones that form an octameric structure required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability and consequently, transcription, and DNA replication and repair. The most widely studied of these modifications are methylation, phosphorylation, and acetylation which may produce different effects on gene regulation depending on the number and pattern of modifications. Histone H2B undergoes acetylation at lysines 5, 15, and 20. It is also phosphorylated in apoptotic cells during DNA fragmentation, possibly in a caspase-dependent manner.

Human recombinant H2B expressed in E. Coli

Application

Chromatin assembly, Western blot (control), Kinase Assay

Physical properties

~17 kDa

Physical form

Liquid in Dulbecco′s PBS. Frozen solution.

Storage and Stability

Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.