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05-166

Sigma-Aldrich

Anti-IGF-II Antibody, clone S1F2

clone S1F2, Upstate®, from mouse

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Synonym(s):
Anti-C11orf43, Anti-GRDF, Anti-IGF-II, Anti-PP9974, Anti-SRS3
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

S1F2, monoclonal

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IGF2(3481)

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This Item
05-172-AF488ABC504I9409
Anti-IGF-II Antibody, clone S1F2 clone S1F2, Upstate®, from mouse

05-166

Anti-IGF-II Antibody, clone S1F2

Anti-IGF2 Antibody from rabbit, purified by affinity chromatography

ABC504

Anti-IGF2 Antibody

species reactivity

human, rat

species reactivity

chicken, rat, mouse, human, fish

species reactivity

human

species reactivity

mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

hamster

UniProt accession no.

P01344

UniProt accession no.

P05019

UniProt accession no.

P01344

UniProt accession no.

P05017

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable, immunocytochemistry: suitable, neutralization: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

western blot: suitable

technique(s)

capture ELISA: 2-8 μg/mL, neutralization: suitable, western blot: 1-2 μg/mL

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General description

Human Insulin-like Growth Factor II (IGFII) contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

Specificity

Less than 10% cross-reactivity to human IGF-I (Tanaka, H, 1989; Suzuki, T, 1989).
This antibody detects IGF-II.

Immunogen

Partially purified rat IGF-II. Clone S1F2

Application

Detect IGF-II using this Anti-IGF-II Antibody, clone S1F2 validated for use in ELISA, IP, IC, NEUT & WB.
Neutralization: 10 μg/mL of a previous lot inhibited the stimulatory effects of IGF-II (10 ng/mL) on DNA synthesis in chicken embryo fibroblasts. Cell viability was assessed using an ATP endpoint assay (ATPLiteÔ-M, Packard Instruments). Previous lots of antibody were tested to inhibit IGF-II-dependent incorporation of [3H]-thymidine
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

Western Blotting Analysis:
1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.

Target description

~7 kDa

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing PBS, pH 7.4., purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Human head and neck tumours, angioma tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Altered nephrogenesis due to maternal diabetes is associated with increased expression of IGF-II/mannose-6-phosphate receptor in the fetal kidney
Amri, K., et al
Diabetes, 50, 1069-1075 (2001)
Immunocytochemical demonstration of IGF-II-like immunoreactivity in human paraganglioma of the craniocervical region
Suzuki, T, et al
Virchows Archiv. A, Pathological Anatomy and Histopathology, 414, 515-521 (1989)
Marcel Trautmann et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 23(20), 6227-6238 (2017-06-24)
Purpose: Myxoid liposarcoma is an aggressive disease with particular propensity to develop hematogenic metastases. Over 90% of myxoid liposarcoma are characterized by a reciprocal t(12;16)(q13;p11) translocation. The resulting chimeric FUS-DDIT3 fusion protein plays a crucial role in myxoid liposarcoma pathogenesis;
Hiroyuki Hirai et al.
Internal medicine (Tokyo, Japan), 55(10), 1309-1314 (2016-05-18)
A 61-year-old woman with multiple metastatic and unresectable gastrointestinal stromal tumors (GISTs) was referred for investigation of refractory hypoglycemia that developed four months before this hospitalization. On admission, her fasting plasma glucose was 38 mg/dL despite 10% glucose infusion. Investigations
S-B Catrina et al.
British journal of cancer, 92(8), 1467-1474 (2005-04-07)
Kaposi's sarcoma (KS) is a highly vascular tumour and is the most common neoplasm associated with human immunodeficiency virus (HIV-1) infection. Growth factors, in particular vascular endothelial growth factor (VEGF), have been shown to play an important role in its

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