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Key Documents

05-253MG

Sigma-Aldrich

Anti-ZAP-70 Antibody, clone 2F3.2

clone 2F3.2, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2F3.2, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_001079

UniProt accession no.

P43403

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ZAP70(7535)

General description

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Specificity

human ZAP-70, does not recognize murine ZAP-70

Immunogen

GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254

Application

Detect ZAP-70 using this Anti-ZAP-70 Antibody, clone 2F3.2 validated for use in FC, IH, IP & WB.
Research Category
Signaling
Research Sub Category
Immunological Signaling

Quality

routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cell lysate

Target description

70kDa

Physical form

Format: Purified
PBS
Protein G Chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marta Crespo et al.
The New England journal of medicine, 348(18), 1764-1775 (2003-05-02)
The mutational status of immunoglobulin heavy-chain variable-region (IgVH) genes in the leukemic cells of chronic lymphocytic leukemia (CLL) is an important prognostic factor in the disease. We investigated whether the expression of ZAP-70 by CLL cells correlated with the IgVH
TCR signaling: another Abl-bodied kinase joins the cascade.
Wange, Ronald L
Current Biology, 14, R562-R564 (2004)
D H Chu et al.
Immunological reviews, 165, 167-180 (1998-12-16)
The processes of T-cell development and activation employ similar immature and mature receptors as well as similar signal transduction pathways to achieve different outcomes. Many signaling molecules are shared between the receptor signaling pathways, including two families of cytoplasmic protein

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