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05-389-AF647

Sigma-Aldrich

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor 647 Conjugate

clone 23A8, from mouse, ALEXA FLUOR 647

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Synonym(s):
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, Rac1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

23A8, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RAC1(5879)

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This Item
05-389-AF48816-31907-896-I
antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

23A8, monoclonal

clone

23A8, monoclonal

clone

23A8, monoclonal

clone

polyclonal

Gene Information

human ... RAC1(5879)

Gene Information

human ... RAC1(5879)

Gene Information

human ... RAC1(5879)

Gene Information

human ... RAC1(5879)

species reactivity

mouse, rat, human

species reactivity

rat, mouse, human

species reactivity

human, rat, mouse

species reactivity

human

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General description

Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.

Specificity

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

Immunogen

Recombinant protein containing the full length human Rac.

Application

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.
Research Category
Signaling
Research Sub Category
Developmental Signaling
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in HeLa cells.

Target description

~21 kDa observed

Physical form

Affinity purified
Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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