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09-271

Sigma-Aldrich

Anti-Rac1b Antibody

Upstate®, from rabbit

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Synonym(s):
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RAC1(5879)

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05-389-AF48816-31907-896-I
Anti-Rac1b Antibody Upstate®, from rabbit

Sigma-Aldrich

09-271

Anti-Rac1b Antibody

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

23A8, monoclonal

clone

23A8, monoclonal

clone

polyclonal

species reactivity

mouse, human

species reactivity

rat, mouse, human

species reactivity

human, rat, mouse

species reactivity

human

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

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General description

Ras-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Specificity

This rabbit polyclonal antibody specifically detects Rac1b in human and murine cells.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Rac1b.

Application

Anti-Rac1b Antibody, Cat. No. 09-271, is a rabbit polyclonal antibody that detects Ras-related C3 botulinum toxin substrate 1 (Rac1b) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Rac1b in HeLa, A431, HUVEC, and NIH/3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.

Quality

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.

Target description

~17 kDa observed; 23.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Ibuprofen inhibits colitis-induced overexpression of tumor-related Rac1b.
Matos, P; Kotelevets, L; Goncalves, V; Henriques, AF; Henriques, A; Zerbib, P; Moyer et al.
Neoplasia null
Huizi Wu et al.
International journal of cancer, 143(11), 2962-2972 (2018-08-16)
Recent studies suggest that malignant melanoma heterogeneity includes subpopulations of cells with features of multipotent neural crest (NC) cells. Zebrafish and mouse models have shown that reactivation of neural crest-specific pathways during transformation determines the invasiveness of melanoma cells. In
Christine Mehner et al.
Genes & cancer, 6(11-12), 480-489 (2016-01-26)
Breast, lung, and pancreatic cancers collectively represent one third of all diagnosed tumors and are responsible for almost 40% of overall cancer mortality. Despite improvements in current treatments, efforts to develop more specific therapeutic options are warranted. Here we identify
Hendrik Ungefroren et al.
Cancers, 11(5) (2019-05-22)
The small GTPase Ras-related C3 botulinum toxin substrate 1B (RAC1B) has been shown previously by RNA interference-mediated knockdown (KD) to function as a powerful inhibitor of transforming growth factor (TGF)-β1-induced cell migration and epithelial-mesenchymal transition in epithelial cells, but the
Matrix metalloproteinase induction of Rac1b, a key effector of lung cancer progression.
Stallings-Mann, ML; Waldmann, J; Zhang, Y; Miller, E; Gauthier, ML; Visscher, DW; Downey et al.
Science Translational Medicine null

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