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05-928

Sigma-Aldrich

Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

clone A3S, Upstate®, from rabbit

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Synonym(s):
H3, Histone H3, H3 histone, family 3A
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

A3S, monoclonal

species reactivity

Saccharomyces cerevisiae, mouse, chicken, yeast, human, rat

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... H3F3B(3021)

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vibrant-m

07-690

Anti-Histone H3 Antibody, CT, pan

antibody form

purified antibody

antibody form

culture supernatant

antibody form

serum

antibody form

culture supernatant

species reactivity

Saccharomyces cerevisiae, mouse, chicken, yeast, human, rat

species reactivity

human, rat, Saccharomyces cerevisiae, mouse, chicken

species reactivity

human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae

species reactivity

human

Gene Information

human ... H3F3B(3021)

Gene Information

human ... H3F3B(3021)

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)

Gene Information

human ... HIST1H3F(8968)

clone

A3S, monoclonal

clone

A3S, monoclonal

clone

polyclonal

clone

MC463, monoclonal

UniProt accession no.

Q16695

UniProt accession no.

Q16695

UniProt accession no.

Q16695

UniProt accession no.

Q16695

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General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Broad species cross-reactivity expected due to sequence homology.
Recognizes Histone H3, Mr 17 kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.

Application

Chromatin Immunoprecipitation:
2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Use Anti-Histone H3 Antibody, CT, pan, clone A3S (Rabbit Monoclonal Antibody) validated in ChIP, WB to detect Histone H3 also known as Histone H3.

Quality

Routinely evaluated by western blot analysis.

Western Blot Analysis:
1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Target description

17 kDa

Physical form

Format: Purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Control
Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle.
Vanagas, L; Dalmasso, MC; Dubremetz, JF; Portiansky, EL; Olins, DE; Angel, SO
Parasitoloty null
Soraya Bravo et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 21(7), 1403-1412 (2013-05-29)
Cancer development involves changes driven by the epigenetic machinery, including nucleosome positioning. Recently, the concept that adenoviral replication may be driven by tumor specific promoters (TSPs) gained support, and several conditionally replicative adenoviruses (CRAd) exhibited therapeutic efficacy in clinical trials.
Claudio D'Addario et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(3), 1069-1075 (2010-11-26)
Ethanol alters neural activity through interaction with multiple neurotransmitters and neuromodulators. The endogenous opioid system seems to play a key role, since the opioid receptor antagonist naltrexone (ReVia®) attenuates craving for alcohol. We recently reported that ethanol and acetaldehyde, the
Vijay S Thakur et al.
Carcinogenesis, 33(2), 377-384 (2011-11-25)
Green tea polyphenols (GTPs) reactivate epigenetically silenced genes in cancer cells and trigger cell cycle arrest and apoptosis; however, the mechanisms whereby these effects occur are not well understood. We investigated the molecular mechanisms underlying the antiproliferative effects of GTP
Renata Picciani et al.
Molecular vision, 14, 871-877 (2008-05-21)
We present a novel and simple enrichment strategy to capture trabecular meshwork (TM) protease proteome. The method relies on fractionation of TM tissue into cytosolic and nuclear extracts and subsequent affinity enrichment of proteases on peptide inhibitors. A large repertoire

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