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06-1398

Sigma-Aldrich

Anti-CDK5RAP2 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

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This Item
05-1592SAB1300900SAB4503203
Anti-CDK5RAP2 Antibody from rabbit, purified by affinity chromatography

06-1398

Anti-CDK5RAP2 Antibody

Anti-mTOR Antibody, clone 21D8.2 clone 2ID8.2, from mouse

05-1592

Anti-mTOR Antibody, clone 21D8.2

clone

polyclonal

clone

2ID8.2, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

mouse, human

species reactivity

human

species reactivity

mouse, rat, human

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... CDK5RAP2(55755)

Gene Information

human ... MTOR(2475)

Gene Information

human ... CDK5R2(8941)

Gene Information

human ... CDK5RAP3(80279)

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:100, indirect ELISA: 1:1000

technique(s)

ELISA: 1:5000, western blot: 1:500-1:1000

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General description

Also known as Cep215, CDK5RAP2 is involved in a variety of cellular processes. It serves as a potential regulator of CDK5 activity through its interaction with CDK5R1. It acts as a negative regulator of centriole disengagement, which maintains centriole engagement and cohesion. It is involved in the regulation of mitotic spindle orientation. Additionally, this protein mediates spindle checkpoint activation by serving as a transcriptional regulator of the MAD2 and BUBR1 promoter. In conjuction with MAPRE1, CDK5RAP2 promotes microtubule polymerization, bundle formation, and growth at the plus ends. It also plays a role in DNA damage-induced G2 cell cycle checkpoint. CDK5RAP2 is known to be mutated in primary microcephaly, a neuodevelopmental disorder that is characterized by a decrease in brain size.

Specificity

Other Homologies: Rat (83% sequence homology) and Mouse (82% sequence homology).

Immunogen

MBP-tagged recombinant protein corresponding to human CDK5RAP2.

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected CDK5RAP2 in RPE1 cells. (Barr, A.R., et al. (2010). J Cell Biol. 189(1):23-39.)
Use Anti-CDK5RAP2 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect CDK5RAP2 also known as CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215.

Quality

Evaluated by Western Blot in 10 µg of HeLa cell lysate.

Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.

Target description

~220 kDa observed. Uniprot.org states four isoforms produced by alternative splicing, which may be observed at a molecular weight of ~216 kDa, ~211 kDa, ~210 kDa, and ~206 kDa in some cell lysates.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Andrew Muroyama et al.
The Journal of cell biology, 213(6), 679-692 (2016-06-15)
Differentiation induces the formation of noncentrosomal microtubule arrays in diverse tissues. The formation of these arrays requires loss of microtubule-organizing activity (MTOC) at the centrosome, but the mechanisms regulating this transition remain largely unexplored. Here, we use the robust loss
Joo-Hee Sir et al.
The Journal of cell biology, 203(5), 747-756 (2013-12-04)
Most animal cells contain a centrosome, which comprises a pair of centrioles surrounded by an ordered pericentriolar matrix (PCM). Although the role of this organelle in organizing the mitotic spindle poles is well established, its precise contribution to cell division
Kaitlin Ching et al.
eLife, 11 (2022-04-15)
Olfactory sensory neurons (OSNs) in vertebrates detect odorants using multiple cilia, which protrude from the end of the dendrite and require centrioles for their formation. In mouse olfactory epithelium, the centrioles originate in progenitor cells near the basal lamina, often
Alexis R Barr et al.
The Journal of cell biology, 189(1), 23-39 (2010-04-07)
The centrosomal protein, CDK5RAP2, is mutated in primary microcephaly, a neurodevelopmental disorder characterized by reduced brain size. The Drosophila melanogaster homologue of CDK5RAP2, centrosomin (Cnn), maintains the pericentriolar matrix (PCM) around centrioles during mitosis. In this study, we demonstrate a
Sadanori Watanabe et al.
The Journal of cell biology, 219(12) (2020-11-11)
Centrosomes, composed of centrioles that recruit a pericentriolar material (PCM) matrix assembled from PCNT and CDK5RAP2, catalyze mitotic spindle assembly. Here, we inhibit centriole formation and/or remove PCNT-CDK5RAP2 in RPE1 cells to address their relative contributions to spindle formation. While

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