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ABT59

Sigma-Aldrich

Anti-Pericentrin Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Pericentrin, Kendrin, Pericentrin-B
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PCNT(5116)

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This Item
HPA016820ABE1856MAB2616
Anti-Pericentrin Antibody from rabbit, purified by affinity chromatography

ABT59

Anti-Pericentrin Antibody

Anti-CEP152 Antibody, N-Term from rabbit

ABE1856

Anti-CEP152 Antibody, N-Term

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500

technique(s)

electron microscopy: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

Gene Information

human ... PCNT(5116)

Gene Information

human ... PCNT(5116)

Gene Information

human ... CEP152(22995)

Gene Information

human ... FERMT1(55612)

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

4A5.14, monoclonal

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General description

Pericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.

Specificity

Other homologies: Mouse (89% sequence homology). No homology to Rat was found.

Immunogen

KLH-conjugated linear peptide corresponding to human Pericentrin.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pericentrin in HeLa cells.
This Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.

Target description

~250 kDa observed.
Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Claudia Conte et al.
Biology open, 7(5) (2018-04-25)
Dynein is the sole processive minus-end-directed microtubule motor found in animals. It has roles in cell division, membrane trafficking, and cell migration. Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and
Alyssa C Rezey et al.
Scientific reports, 9(1), 7555-7555 (2019-05-19)
Allergic asthma is characterized by airway smooth muscle layer thickening, which is largely attributed to cell division that requires the formation of centrosomes. Centrosomes play a pivotal role in regulating bipolar spindle formation and cell division. Before mitosis, centrosomes undergo
Ting Lin et al.
Molecular medicine reports, 16(6), 8685-8690 (2017-10-11)
Previous studies have indicated that glomerular podocyte injury serves a crucial role in proteinuria during the process of chronic kidney disease. The slit diaphragm of podocytes forms the final barrier to proteinuria. Dendrin, a constituent of the slit diaphragm protein
Anke Brüning-Richardson et al.
Cancers, 13(23) (2021-12-11)
Previous data on glycogen synthase kinase 3 (GSK-3) inhibition in cancer models support a cytotoxic effect with selectivity for tumor cells compared to normal tissue but the effect of these inhibitors in glioma has not been widely studied. Here, we
Qian Wang et al.
The Journal of biological chemistry, 292(31), 12874-12884 (2017-06-18)
The X-linked deubiquitinase USP9X has been implicated in multiple pathological disorders including malignancies and X-linked intellectual disability. However, its biological function and substrate repertoire remain to be investigated. In this study, we utilized the tandem mass tag labeling assay to

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