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06-562

Sigma-Aldrich

Anti-erbB-2/HER-2 Antibody

Upstate®, from rabbit

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Synonym(s):
CD340 antigen, CD340 antigen, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, c-erb B2/neu protein, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog),
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

monkey, human, mouse

species reactivity (predicted by homology)

rat

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ERBB2(2064)
mouse ... Erbb2(13866)
rat ... Erbb2(24337)
rhesus monkey ... Erbb2(697573)

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This Item
06-229MABE330SAB5700151
vibrant-m

06-562

Anti-erbB-2/HER-2 Antibody

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

species reactivity

monkey, human, mouse

species reactivity

human, rat

species reactivity

human

species reactivity

rat, human, mouse

isotype

IgG

isotype

IgG

isotype

IgG1κ

isotype

-

NCBI accession no.

NM_004448

NCBI accession no.

NM_004448

NCBI accession no.

NP_001005862

NCBI accession no.

-

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General description

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Specificity

Recognizes c-erbB-2 (185 kDa), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.

Immunogen

A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein.

Application

Anti-erbB-2/HER-2 Antibody is an antibody against erbB-2/HER-2 for use in IH, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated erbB-2/HER-2 from 600 μg MCF-7 RIPA lysate, and previously in A431, LNCaP, and c-erbB-2 transfected 3T3/NIH RIPA lysate.

Immunohistochemistry:
A previous lot of antibody has been reported by an independent laboratory, to detect erbB-2/HER-2 in frozen and formalin/paraffin sections of rat breast.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Quality

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Her-2 on 10 μg of HEK293 lysates.

Target description

185 kDa

Linkage

Replaces: 04-1127

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
A431 cells, HEK293, Jurkat, MCF7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Introducing the cancer microenvironment section of Journal of Translational Medicine.
Fernando Vidal-Vanaclocha,Isaac P Witz
Journal of Translational Medicine null
Pathologic findings from the National Surgical Adjuvant Breast and Bowel Project: prognostic significance of erbB-2 protein overexpression in primary breast cancer.
Paik, S, et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 8, 103-112 (1990)
Different mechanisms are implicated in ERBB2 gene overexpression in breast and in other cancers.
Vernimmen, D; Gueders, M; Pisvin, S; Delvenne, P; Winkler, R
British Journal of Cancer null
Concomitant targeting of EGF receptor, TGF-beta and SRC points to a novel therapeutic approach in pancreatic cancer.
Deharvengt, S; Marmarelis, M; Korc, M
Testing null
Valentina Pirazzoli et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(2), 426-435 (2015-09-06)
The EGFR tyrosine kinase inhibitors (TKIs), erlotinib and afatinib, have transformed the treatment of advanced EGFR-mutant lung adenocarcinoma. However, almost all patients who respond develop acquired resistance on average approximately 1 year after starting therapy. Resistance is commonly due to

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