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06-935

Sigma-Aldrich

Anti-Estrogen Receptor α Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (immunogen homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ESR1(2099)
mouse ... Esr1(13982)
rat ... Esr1(24890)

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This Item
04-156404-82004-824
Anti-Estrogen Receptor α Antibody from rabbit, purified by affinity chromatography

06-935

Anti-Estrogen Receptor α Antibody

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

culture supernatant

antibody form

culture supernatant

clone

polyclonal

clone

F3-A, monoclonal

clone

60C, monoclonal

clone

68-4, monoclonal

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

UniProt accession no.

P06211

UniProt accession no.

P03372

UniProt accession no.

P03372

UniProt accession no.

P03372

Gene Information

human ... ESR1(2099)
mouse ... Esr1(13982)
rat ... Esr1(24890)

Gene Information

human ... ESR1(2099)

Gene Information

bovine ... Esr1(407238)
human ... ESR1(2099)
mouse ... Esr1(13982)
pig ... Esr1(397435)
rat ... Esr1(24890)

Gene Information

human ... ESR2(2100)
mouse ... Esr2(13983)
rat ... Esr2(25149)

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General description

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

Specificity

This antibody recognizes the C-terminus of Estrogen Receptor alpha.

Immunogen

Epitope: C-terminus.
KLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.

application

  • Tested applications
  • Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor in human stomach, human uterine cancer, human breast cancer and human cervical cancer tissue sections.
  • Immunoprecipitation Analysis: 4 µg from a representative lot immunoprecipitated Estrogen Receptor in MCF-7 cell lysate.
  • Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Estrogen Receptor in MCF-7 cells.
  • Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. "
Anti-Estrogen Receptor , Cat. No. 06-935, is a rabbit polyclonal antibody that detects Estrogen Receptor and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Immunohistochemistry (Paraffin).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Hormones & Receptors

Quality

Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor in MCF-7 cell lysate.

Target description

~58 kDa observed. UniProtKB/Swiss-Prot P06211 (ESR1_RAT) lists a molecular weight of 67 kDa A molecular weight between 52-58 kDA due to variant isoforms may be observed in some cell lysates (Al-Bader, M.D. (2006). Reprod Biol Endocrinol. 4:13.; Geisler, J.G. et al. (2002). Diabetes. 51(7):2158-2169.). Uncharacterized bands may be seen at a molecular weight of ~10 kDa, ~35 kDa, ~41 kDa, ~105 kDa, and ~220 kDa in some cell lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Specific modulation of estrogen receptor mRNA isoforms in rat pituitary throughout the estrous cycle and in response to steroid hormones.
Friend, K E, et al.
Molecular and cellular endocrinology, 131, 147-155 (1997)
Transcriptional regulation by a naturally occurring truncated rat estrogen receptor (ER), truncated ER product-1 (TERP-1).
Schreihofer, D A, et al.
Molecular Endocrinology, 13, 320-329 (1999)
Neurochemical characterization and sexual dimorphism of projections from the brain to abdominal and subcutaneous white adipose tissue in the rat.
Adler, ES; Hollis, JH; Clarke, IJ; Grattan, DR; Oldfield, BJ
The Journal of Neuroscience null
Differential expression of oestrogen receptor alpha following reproductive experience in young and middle-aged female rats.
E M Byrnes,J A Babb,R S Bridges
Journal of Neuroendocrinology null
The impact of neonatal bisphenol-A exposure on sexually dimorphic hypothalamic nuclei in the female rat.
Adewale, HB; Todd, KL; Mickens, JA; Patisaul, HB
Neurotoxicology null

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