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Sigma-Aldrich

Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
JNK1 alpha protein kinase, JNK1 beta protein kinase, JUN N-terminal kinase, Stress-activated protein kinase JNK1, Stress-activated protein kinase JNK1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-ac
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, horse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

activity assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (p(Thr183/pTyr185), (pThr221/pTyr223))

Gene Information

human ... MAPK8(5599)

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Anti-c-Jun Antibody, clone 6E4.4 clone 6E4.4, from mouse

05-1076

Anti-c-Jun Antibody, clone 6E4.4

clone

polyclonal

clone

C05T, monoclonal

clone

6E4.4, monoclonal

clone

polyclonal

species reactivity

human, mouse, horse

species reactivity

rat, human

species reactivity

mouse, human, rat

species reactivity

rat, human, mouse

antibody form

affinity isolated antibody

antibody form

culture supernatant

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Gene Information

human ... MAPK8(5599)

Gene Information

human ... MAPK10(5602)
rat ... Mapk10(25272)

Gene Information

human ... JUN(3725)
mouse ... Jun(16476)
rat ... Jun(24516)

Gene Information

human ... MAP2K4(6416)

UniProt accession no.

P45983

UniProt accession no.

P49187

UniProt accession no.

P45983

UniProt accession no.

P45985

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General description

JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.

Specificity

This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .

Immunogen

Epitope: Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223
KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.

Application

Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.
Research Category
Signaling
Research Sub Category
MAP Kinases

Quality

Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.

Target description

~ 42 kDa observed. There are several isoforms between 40-50 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated and Lambda phosphatase treated RAW264.7 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Vinicius Toledo Ribas et al.
PloS one, 7(4), e34483-e34483 (2012-04-13)
Most studies of c-Jun N-terminal Kinase (JNK) activation in retinal tissue were done in the context of neurodegeneration. In this study, we investigated the behavior of JNK during mitosis of progenitor cells in the retina of newborn rats. Retinal explants
Yao Fong et al.
Cancer cell international, 17, 37-37 (2017-03-14)
2,9-Bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy] phenyl}-11H-indeno[1,2-c]quinoline-11-one (BPIQ), is a synthetic quinoline analog. A previous study showed the anti-cancer potential of BPIQ through modulating mitochondrial-mediated apoptosis. However, the effect of BPIQ on cell migration, an index of cancer metastasis, has not yet been examined. Furthermore
CD22 regulates adaptive and innate immune responses of B cells.
Kawasaki, N; Rademacher, C; Paulson, JC
Journal of Innate Immunity null
Cardiotoxin III inhibits proliferation and migration of oral cancer cells through MAPK and MMP signaling.
Yen, CY; Liang, SS; Han, LY; Chou, HL; Chou, CK; Lin, SR; Chiu, CC
TheScientificWorldJournal null
Jennifer M Rutkowsky et al.
American journal of physiology. Endocrinology and metabolism, 306(12), E1378-E1387 (2014-04-25)
Incomplete β-oxidation of fatty acids in mitochondria is a feature of insulin resistance and type 2 diabetes mellitus (T2DM). Previous studies revealed that plasma concentrations of medium- and long-chain acylcarnitines (by-products of incomplete β-oxidation) are elevated in T2DM and insulin

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