07-224
Anti-phospho-STAT2 (Tyr689) Antibody
Upstate®, from rabbit
Synonym(s):
Anti-Anti-IMD44, Anti-Anti-ISGF-3, Anti-Anti-P113, Anti-Anti-PTORCH3, Anti-Anti-STAT113
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr689)
Gene Information
human ... STAT2(6773)
General description
Signal transducer and activator of transcription 2 (UniProt: Q9WVL2; also known as STAT2) is encoded by the Stat2 gene (Gene ID: 20847) in murine species. The JAK-STAT signaling pathway play an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. In mammals, seven different STATs have been recognized. They contain a conserved tyrosine residue near the C-terminus, which is phosphorylated by JAKs, which allows the dimerization of STATs by interacting with a conserved SH2 domain. In their latent form STATs are located in the cytoplasm and upon phosphorylation they are transported into the nucleus by an importin -5 dependent mechanism. In the nucleus, dimerized STATs bind to specific regulatory sequences to activate or repress transcription of target genes. The main function of STAT2 is to mediate IFN- /β signaling. Following type I interferon binding to cell surface receptors, JAK kinases are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes. STAT2 is also reported to serve as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at serine 616 and serine 637, which activate and inactivate the GTPase activity of DNM1L respectively. (Ref.: Lee, CJ., et al. (2020). Exp. Mol. Med. 52(9); 1526-1536; Sahani, R., et al. (2015). Brain. 138(10); 2834-2846; Bluyssen, HA., and Levy, DE. (1997). 272(7); 4600-4605).
Specificity
This rabbit polyclonal antibody detects STAT2 phosphorylated on tyrosine 689.
Immunogen
KLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphotyrosine 689 from the C-terminal half of mouse STAT2.
Application
Tested Applications
- Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho-STAT2 (Tyr689) in lysate from HeLa cells stimulated with Interferon and Interferon
- Peptide Inhibition Assay: Target band detection in lysate from HeLa cells stimulated with Interferon and Interferon was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-phospho-STAT2 (Tyr689), Cat. No. 07-224, is a rabbit polyclonal antibody that detects STAT2 phosphorylated on tyrosine 689 and is tested for use in Immunoprecipitation, Peptide Inhibition, and Western Blotting.
Quality
Evaluated by Western Blotting in HeLa cells stimulated with Interferon and .Western Blotting Analysis (WB): A 1:200 dilution of this antibody detected STAT2 phosphorylated on tyrosine 689 in HeLa cells stimulated with Interferon (0.3 ng/mL for 16 h) and Interferon ( 2000 U/mL for 20 min).
Target description
~110 kDa observed; 105.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Analysis Note
Control
IFN-gamma then IFN-alpha treated HeLa cell lysate
IFN-gamma then IFN-alpha treated HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
A human cytomegalovirus antagonist of type I IFN-dependent signal transducer and activator of transcription signaling.
Paulus, C; Krauss, S; Nevels, M
Proceedings of the National Academy of Sciences of the USA null
The STAT2 activation process is a crucial target of Sendai virus C protein for the blockade of alpha interferon signaling.
Bin Gotoh, Kenji Takeuchi, Takayuki Komatsu, Junko Yokoo
Journal of virology null
Rotavirus antagonizes cellular antiviral responses by inhibiting the nuclear accumulation of STAT1, STAT2, and NF-kappaB.
Holloway, Gavan, et al.
Journal of virology, 83, 4942-4951 (2009)
Nagaraj Kerur et al.
Nature medicine, 24(1), 50-61 (2017-11-28)
Geographic atrophy is a blinding form of age-related macular degeneration characterized by retinal pigmented epithelium (RPE) death; the RPE also exhibits DICER1 deficiency, resultant accumulation of endogenous Alu-retroelement RNA, and NLRP3-inflammasome activation. How the inflammasome is activated in this untreatable
Measles virus V protein blocks interferon (IFN)-alpha/beta but not IFN-gamma signaling by inhibiting STAT1 and STAT2 phosphorylation.
Kaoru Takeuchi, Shin-ich Kadota, Makoto Takeda, Naoko Miyajima, Kyosuke Nagata
Febs Letters null
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