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07-841

Sigma-Aldrich

Anti-phospho-IR/IGF1R (Tyr1158/Tyr1162/Tyr1163) Antibody

Upstate®, from rabbit

Synonym(s):

CD220, Insulin Receptor / CD221, Insulin-like Growth Factor 1 Receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr1158/pTyr1162/pTyr1163)

Gene Information

human ... INSR(3643)

Specificity

Human and mouse phospho-Insulin Receptor (IR) Tyrosine 1158/1162/1163 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131/1135/1136. Rat reactivity is expected based on 100% sequence homology.
Predicted to cross-react with rat based on 100% sequence homology, but have not been tested.

Immunogen

Peptide corresponding to the amino acid region encompassing the human, mouse, and rat phospho-Insulin Receptor (IR) Tyrosine 1158, 1162, and 1163 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131, 1135, and 1136.

Application

Anti-phospho-IR/IGF1R (Tyr1158/Tyr1162/Tyr1163) Antibody detects level of phospho-IR/IGF1R (Tyr1158/Tyr1162/Tyr1163) & has been published & validated for use in WB.
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-IR (Tyr1158/Tyr1162/Tyr1163) in RIPA lysate of untreated and insulin treated CHO cells transfected with IR.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Routinely evaluated by immunoblot.

Target description

155 kDa

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified

Storage and Stability

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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