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1.00079

Millipore

Bolton Broth Selective Supplement

according to ISO 10272-1, for Campylobacter spp., pkg of 10 vials, For the preparation of Bolton Selective Enrichment Broth

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Synonym(s):
Campylobacter Selective Supplement

agency

according to ISO 10272-1

Quality Level

reg. compliance

FDA -BAM

form

lyophilized powder

composition

Amphotericin B, 5 mg
Cefoperazone, 10 mg
Trimethoprim, 10 mg
Vancomycin, 10 mg

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

2-8°C

suitability

Campylobacter spp.
Listeria spp.

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This Item
1.101411.000711.07006
Bolton Broth Selective Supplement according to ISO 10272-1, for Campylobacter spp., pkg of 10 vials, For the preparation of Bolton Selective Enrichment Broth

1.00079

Bolton Broth Selective Supplement

Millipore

1.10141

Salmosyst Selective Supplement

CCDA Selective Supplement for Campylobacter spp., pkg of 10 vials, For the preparation of Campylobacter Blood Free Selective Agar

1.00071

CCDA Selective Supplement

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

form

lyophilized powder

form

solid

form

lyophilized powder

form

solid

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.

Application

Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.

Linkage

Replaces: 1.00079.0017

Analysis Note

Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004):
10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004):
>10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023): no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004):
greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156): greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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