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67454

Millipore

Bolton Broth, Base

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Bolton Selective Enrichement Broth, Base
NACRES:
NA.85

agency

according to ISO 10272-1:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

enzymatic digest of animal tissues, 10 g/L
α-ketoglutaric acid, 1.0 g/L
lactalbumin hydrolysates, 5.0 g/L
sodium carbonate, 0.6 g/L
sodium chloride, 5.0 g/L
sodium metabisulfite, 0.5 g/L
sodium pyruvate, 0.5 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
pathogen testing
sample preparation

microbiology

suitability

selective for Campylobacter spp.

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Bolton Broth, Base suitable for microbiology, NutriSelect® Plus

67454

Bolton Broth, Base

Blood Free Campylobacter Broth suitable for microbiology, NutriSelect® Plus

59751

Blood Free Campylobacter Broth

agency

according to ISO 10272-1:2017

agency

-

agency

according to ISO 6888-3:2003

agency

according to ISO 16654:2001

suitability

selective for Campylobacter spp.

suitability

selective for Campylobacter spp.

suitability

Staphylococcus spp.

suitability

selective for Escherichia coli, selective for coliforms, selective for enterovirulent E. coli (EEC)

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

non-sterile

composition

enzymatic digest of animal tissues, 10 g/L , lactalbumin hydrolysates, 5.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 0.5 g/L , α-ketoglutaric acid, 1.0 g/L , sodium metabisulfite, 0.5 g/L , sodium carbonate, 0.6 g/L , yeast extract, 5.0 g/L

composition

beef extract, 10 g/L , casein enzymic hydrolysate, 3 g/L , charcoal bacteriological, 4 g/L , ferrous sulfate, 0.25 g/L , peptic digest of animal tissue, 1 g/L , sodium chloride, 1 g/L , sodium deoxycholate, 1 g/L , sodium pyruvate, 0.25 g/L

composition

beef extract, 5.0 g/L , casein enzymic hydrolysate, 10.0 g/L , glycine, 1.2 g/L , lithium chloride, 5.0 g/L , mannitol, 20.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 3.0 g/L , yeast extract, 5.0 KBE/mL

composition

bile salts No. 3, 1.5 g/L , di-potassium hydrogen phosphate, 4.0 g/L , D(+)-glucose, 2.5 g/L , novobiocin , peptone from casein, 17.0 g/L , peptone from soymeal, 3.0 g/L , sodium chloride, 5.0 g/L

General description

Bolton broth base is used for the selective enrichment of Campylobacter species from foods as recommended by ISO Committee under specifications ISO 10272-1:2006 (E).

Application

for the selective enrichment of Campylobacter species from foods

Preparation Note

Suspend 13.8 grams in 472.5 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1vial of Bolton Selective Supplement (Cat. No. 40568) and 25 ml of sterile lysed defribinated horse blood. Mix well before dispensing into sterile tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Articles

Campylobacter grows only on complex media that have been amended with diverse essential amino acids and supplements. We offer a broad range of specific agars and broths for the detection, identification, and cultivation of Campylobacter.

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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