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Millipore

GranuCult® plus Rose-Bengal Chloramphenicol Agar (RBC)

for yeasts, for molds, suitable for microbiology

Synonym(s):

RBC Agar, Yeast and mold medium

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

granular

manufacturer/tradename

GranuCult® plus

packaging

pkg of -500 g

storage condition

dry media (Tightly closed)

technique(s)

microbiological culture: suitable

color

pink

pH

7.0-7.4 (25 °C, 33.2 g/L in H2O)

solubility

33.2 g/L

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

molds
yeasts

General description

GranuCult® plus Rose-Bengal Chloramphenicol Agar is a selective agar for the enumeration of yeasts and molds in foodstuffs. The neutral pH in combination with chloramphenicol suppresses the growth of most bacteria. Rose-Bengal, taken up intracellular by fungi, restricts the size and the spreading of molds, preventing the overgrowth of slow-growing species by luxuriant species.

Application

GranuCult® plus Rose-Bengal Chloramphenicol Agar is suitable for the enumeration of yeasts and molds in foodstuffs, particularly proteinaceous food.

Features and Benefits

  • GranuCult® offers superior granulated culture media.
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions.
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): turbid
Appearance (colour): pink to red
pH-value (25 °C): 7.0 - 7.4
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): 10 - 100
Inoculum on reference medium (Rhodotorula mucilaginosa DSM 70403): 10 - 100
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Rhodotorula mucilaginosa DSM 70403):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 50 %
Recovery on test medium (Rhodotorula mucilaginosa DSM 70403): ≥ 50 %
Growth (Mucor racemosus ATCC 42647): fair to good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): none
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): none
Incubation: 5 days; 22 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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