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Envirocheck, Contact total viable count coated with Nutrient Agar

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material

plastic cap
plastic slide
plastic tube

Quality Level

manufacturer/tradename

Envirocheck

technique(s)

cell counting: suitable (total viable count)

application(s)

environmental
industrial hygiene
industrial qc
microbiology

storage temp.

2-30°C

suitability

bacteria (total viable count)

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technique(s)

cell counting: suitable (total viable count)

technique(s)

-

technique(s)

-

technique(s)

-

application(s)

environmental
industrial hygiene
industrial qc
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Envirocheck

manufacturer/tradename

Envirocheck

manufacturer/tradename

Envirocheck

manufacturer/tradename

Envirocheck

General description

(Total Viable Counts) Envirocheck
Envirocheck contact total viable count, a semi-quantitative detection of microorganisms on surfaces and in liquids, are flexible paddles that are coated with Nutrient Agar to determine the total viable count of bacteria in sample. Slide A is Coated with Nutrient Agar containing 0.05% Triphenyl Tetrazolium Chloride (TTC) and Slide B Coated with Nutrient Agar. The growth of bacteria on slide A is indicated by red colonies due to the formation of red formazan dye when TTC is reduced.

Application

Envirocheck contact slides can be used for both surface and liquid testing in industrial and environmental monitoring.

Analysis Note

Count the number of colonies on both sides of the paddle- results should not differ by a factor greater than 2, where the colonies on side A will appear mainly in red. In the presence of TTC a few microorganisms may grow less profusely. Always go with the higher counts in case they are not identical on both sides. This represents the microbial load for the test.
Sterility: passes test
pH-value/side one (25 °C): 6.7 - 7.3
pH-value/side two (25 °C): 6.7 - 7.3
Growth/side one
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): fair/good
- Candida albicans ATCC 10231 (WDCM 00054): none/poor
- Bacillus subtilis ATCC 6633 (WDCM 00003): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Growth/side two
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): good
- Candida albicans ATCC 10231 (WDCM 00054): good
- Bacillus subtilis ATCC 6633 (WDCM 00003): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Incubation: 48 hrs.; 35 °C; aerobic; Aspergillus up to 72 hrs.

Legal Information

ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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