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1.03784

Millipore

Egg yolk emulsion

suitable for microbiology, sterile, 50%

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Synonym(s):
Egg yolk suspension, EY broth, egg yolk enrichment broth

biological source

chicken egg yolk

Quality Level

sterility

sterile

form

emulsion

packaging

pkg of 10 × 100 mL

concentration

50%

technique(s)

microbiological culture: suitable

density

1.02 g/cm3

application(s)

microbiology

storage temp.

2-8°C

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This Item
GW1101075208P6386
Millipore

1.03784

Egg yolk emulsion

Sigma-Aldrich

GW11010

IgY from chicken egg yolk

Egg yolk Tellurite Emulsion suitable for microbiology

75208

Egg yolk Tellurite Emulsion

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

emulsion

form

buffered aqueous solution

form

liquid

form

solution

biological source

chicken egg yolk

biological source

-

biological source

-

biological source

egg yolk

sterility

sterile

sterility

-

sterility

sterile

sterility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Egg yolk emulsion is a mixture of egg yolk, typically from chicken eggs, and an aqueous solution, usually saline or phosphate-buffered saline (PBS). It is commonly used in microbiology and related fields as a growth supplement for bacteria and other microorganisms. The emulsion provides a complex mixture of nutrients, including lipids, amino acids, and vitamins, that support the growth of a wide range of microorganisms. Egg yolk emulsion can be added to a variety of microbial culture media to improve the growth of fastidious bacteria that require specific nutrients or additional growth factors. It is particularly useful for the cultivation of bacteria that need cholesterol for growth, such as Mycobacterium species and some of the anaerobic bacteria.

Application

Egg yolk emulsion is commonly used for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species, as well as for the growth of anaerobic bacteria. In addition, egg yolk emulsion can be used as a component of enrichment broths to improve the recovery of pathogens from food samples. Egg yolk emulsion can also be used as an ingredient in selective media formulations, such as egg yolk-polymyxin agar, used for the selective isolation of anaerobic bacteria from various samples.

Linkage

Replaces: 1.03784.0007

Analysis Note

Sterility test: sterile
Typical composition: Sterile egg-yolk 500 ml; NaCl 4.25 g; distilles water to give a final volume of 1000 ml.
Growth promotion test in accordance with ISO 11133.
Egg-yolk Emulsion tested in Mannitol-Egg-yolk-Polymyxine -Agar (=MYP-agar) Art. 1.05267.
Growth
- Bacillus cereus ATCC 11778 (WDCM 00001):
good growth
- Bacillus subtilis ATCC 6633 (WDCM 00003): no limit
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Escherichia coli ATCC 25922 (WDCM 00013): no growth
Colony colour
- Bacillus cereus ATCC 11778 (WDCM 00001):
pink
- Bacillus subtilis ATCC 6633 (WDCM 00003): yellow
Precipitate
- Bacillus subtilis ATCC 6633 (WDCM 00003):
-
- Bacillus cereus ATCC 11778 (WDCM 00001): +
Incubation: 48 hrs. 30 °C; B.cereus 24-48 hrs. 30 °C.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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