Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

1.03840

SAFC

Cellvento® 4CHO-X COMP

expansion medium

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106213

Quality Level

500

description

shipped with cooling packs

product line

Cellvento®

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

storage temp.

2-8°C

Related Categories

General description

There are several advantages to using perfusion in your seed train, namely, flexibility in your production processes, increase in capacity and footprint reduction. Reducing the number of bioreactors in your seed train leads to a better utilization and eliminates traditional bottlenecks (compressed seed train). Additionally, using N-1 perfusion enables the inoculation of a production bioreactor with higher cell densities for high seed production process formats. Cellvento® 4CHO-X Expansion Medium has been specifically developed for N-1 perfusion for Chinese Hamster Ovary (CHO) cell lines. It is formulated with a high nutrient concentration to achieve low cell specific perfusion rates (CSPRs) at high cell densities. The medium is chemically defined, of non-animal origin, and contains no hydrolysates or components of unknown composition.

Application

Cellvento® 4CHO-X Expansion Medium has been specifically formulated to control the metabolic profile of the cells, both to minimize toxic byproducts and to ensure adequate nutrient levels for high cell densities. Preventing nutrient depletion can increase robustness and predictability of seed train operations. This medium supports high growth and viability, thus increasing cell biomass for cryopreservation or inoculation of another reactor. The medium addresses seed train intensification and shows a positive impact in IgG production, when combined in perfusion production with EX-CELL® Advanced HD Perfusion medium (24370C), and in Fed-batch production with e.g. Cellvento® 4CHO medium (103795) and Cellvento® 4Feed (103796). It is expected to work with other media platforms as well. Cellvento® 4CHO-X Expansion Medium can be used for all seed train expansion steps including N-1, reaching robust growth at low CSPRs. This product is intended for research or further manufacturing but not for human or therapeutic use.

Quantity

Formulated to contain 26.2 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Reproducible Scaling of a Cell Retention Filter Using the Cellicon® Cell Retention Solution

This page describes the design attributes of the Cellicon® filter assembly for cell retention and how they simplify scale-up of intensified upstream processes.

Operational and Financial Advantages of Cellicon® Cell Retention Solution in an N-1 Perfusion Process

This page describes the financial and operational advantages offered by the Cellicon® Cell Retention Device in an N-1 perfusion process at the 2,000 L scale in comparison to a cell retention device from another supplier.

Seed Train Intensification Using High Cell Density Cryopreservation

High cell density cryopreservation (HCDC) is a closed processing alternative to the traditional seed train expansion process. Learn more about the benefits of HCDC.

Establishing the First GMP Guideline for Cell Culture Media

Cell culture media are vital for bioprocessing therapeutics. This page describes the central role of our organization in authoring purpose-built GMP for cell culture media.

See All

Related Content

Seed Train Intensification

Biopharmaceutical developers need efficient, productive upstream processes that deliver high quality monoclonal antibodies (mAbs) and other types of recombinant proteins. Process intensification can future-proof their manufacturing strategies, reduce costs, and increase facility flexibility.

Production and Harvest Intensification

Biopharmaceutical developers need efficient, productive upstream processes that deliver high quality monoclonal antibodies (mAbs) and other types of recombinant proteins. Process intensification can future-proof their manufacturing strategies, reduce costs, and increase facility flexibility.

Accelerate development and optimization of cell culture media for upstream perfusion processes

This page describes the use of a small-scale perfusion bioreactor with integrated microfluidic flow control and sensor technologies to predict large-scale processes.

Cell Culture Media Development for mAbs

Learn more about cell culture media development for mAB development and manufacturing, and the products you need for this step.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service