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1.04140

Millipore

Singlepath® Salmonella

input: food(s), suitable for lateral flow assay, for Salmonella spp.

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Synonym(s):
Rapid Salmonella Food Test

agency

according to AOAC® PTM

Quality Level

shelf life

15 mo.

packaging

pack of 25 strips

manufacturer/tradename

Singlepath®

technique(s)

lateral flow assay: suitable
microbe id | immunological: suitable

input

food(s)

application(s)

food and beverages
pathogen testing

storage temp.

2-8°C

suitability

Citrobacter spp.
Salmonella spp.

General description

Singlepath® Salmonella is a rapid diagnostic test, designed to detect Salmonella bacteria in various food samples. Salmonella is a genus of bacteria that can cause human infections, leading to symptoms such as diarrhea, fever, and abdominal cramps.The Singlepath® Salmonella is an immunochromatographic rapid test based on gold-labeled antibodies to detect the presence of a specific antigen, in this case, the Salmonella bacteria. The test includes a nitrocellulose membrane strip that is coated with antibodies specific to Salmonella antigens. When the sample is applied to the test strip, any Salmonella antigens present will bind to the antibodies on the strip, resulting in the development of a visible test line. The test takes approximately 20 minutes to complete, and the results are interpreted based on the presence or absence of the test line. The detection limit can be regarded as less than one colony-forming unit in a 25 g food sample, depending on the serogroup. No additional equipment expenditure is needed.

Application

SINGLEPATH® Salmonella is an immunochromatographic rapid test intended for use in microbiology laboratories analyzing food and animal feeds for the presumptive qualitative detection of Salmonella spp. from food matrices such as meats (raw ground beef and raw ground turkey), spices (black pepper), dairy (dried skimmed milk), dried foods (coconut) and seafood (cooked, peeled frozen prawns).

Features and Benefits

  • Rapid and reliable method
  • High sensitivity and specificity compared to conventional culture methods
  • Can be performed without the need for specialized equipment or personnel.

Linkage

Replaces: 1.04140.0002

Analysis Note

Performance test (Salmonella enteritidis NCTC 5188 (WDCM 00119)): +
Performance test (Salmonella typhimurium ATCC 14028 (WDCM 00031)): +
Performance test (Escherichia coli ATCC 25922 (WDCM 00013)): -
Performance test (Citrobacter freundii ATCC 8090): -

Legal Information

AOAC is a registered trademark of AOAC International
SINGLEPATH is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids


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Articles

Salmonella pass through the entire food chain from animal feed, primary production, and into households or food-service establishments.

Related Content

Detect pathogens rapidly using our lateral flow immunological tests which act as mini-laboratories for identifying the presence of specific pathogens.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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