1.10266

GranuCult^® prime Lauryl Sulfate broth

according to ISO 4831, ISO 7251, FDA-BAM, suitable for microbiology, for Escherichia coli

• Synonym(s): Coliform selective medium, Lauryl Sulfate broth (Granulated)
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: SMWWPart 9221; FDA-BAM; GB 4789.38-2012; ISO 7251; according to ISO 4831
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 5 kg; pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 6.8 (35.5 g/L in H₂O); 6.8±0.2 (25 °C)
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Escherichia coli

Description

General description

GranuCult^® prime Lauryl Sulfate broth (acc. ISO 4831, ISO 7251, and FDA-BAM) is used to selectively enrich and detect coliforms from food and animal feed, water, and other materials. The high nutrient quality and the presence of phosphate buffer ensure rapid growth and increased gas production of even the "slowly lactose-fermenting" coliform bacteria. Gas formation can be detected by using Durham tubes. The lauryl sulfate inhibits the growth of undesired bacteria.

Application

GranuCult^® prime Lauryl Sulfate broth is suitable for the selective enrichment and presumptive detection of coliform bacteria from food and animal feed, water, and other materials.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Linkage

Replaces: 1.10266.5007

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): >10⁴
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): >10⁴
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): none
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): none
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Incubation:
Escherichia coli: 24 ± 2 hours at 30 ± 1 °C and parallel 24 ± 2 hours at 37 ± 1 °C
Citrobacter: 24 ± 2 hours at 30 ± 1 °C
Enterococcus faecalis: 48 ± 2 hours at 30 ± 1 °C and parallel 48 ± 2 hours at 37 ± 1 °C

Other Notes

• The prepared medium is clear and yellowish-brown
• Store the prepared medium in screw-capped containers at 2 °C to 8 °C in the dark and for up to one month

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2