1.10765

EC (Escherichia coli) broth

GranuCult^® prime, suitable for microbiology, for Escherichia coli

• Synonym(s): E. coli selective medium, Escherichia coli broth, EC broth
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: FDA-BAM; ISO 7251
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• storage condition: dry media (Tightly closed.)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 6.7-7.1 (25 °C, 37 g/L in H₂O, after autoclaving)

Description

General description

Escherichia coli broth is suitable for the selective enrichment, enumeration, and confirmation of coliform bacteria. The lactose content of this medium favors the growth of lactose-positive bacteria, especially coliform bacteria, and E. coli. The bile salts inhibit the growth of Gram-positive bacteria or microorganisms that are not adapted to the intestinal environment. Lactose-positive bacteria metabolize lactose with gas formation.

Application

Escherichia coli broth is suitable for the selective enrichment, enumeration, and confirmation of coliform bacteria from food and animal feed, and other materials.

Features and Benefits

• GranuCult^® prime offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1E+04
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1E+04
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Escherichia coli: 24 ± 2 hours at 44 ± 1 °C
other teststrains: 48 ± 2 hours at 44 ± 1 °C

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® prime and the cost-efficient powdered^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2