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GranuCult® prime Sodium chloride peptone broth (buffered)

according to EP, USP, JP, for aerobic bacteria, suitable for microbiology

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Synonym(s):
Buffered NaCl-Peptone Broth, Buffered NaCl-Peptone Solution, Buffered Sodium Chloride Peptone Solution, Buffered sodium chloride peptone broth

agency

JP
USP
according to EP

Quality Level

form

medium granules (dehydrated (DCM))

packaging

pkg of 5 kg
pkg of 500 g

manufacturer/tradename

GranuCult® prime

storage condition

protect from light (Keep dry and tightly closed)

technique(s)

microbiological culture: suitable

color

light brown

pH

6.8-7.2 (25 °C, 16.1 g/L in H2O, after autoclaving)

solubility

16.1 g/L

application(s)

microbiology
pharmaceutical

storage temp.

15-25°C

suitability

aerobic bacteria

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This Item
1.072281.004661.00465
agency

JP, according to EP, USP

agency

APHA, EP, ISO 19250, ISO 21528-1, ISO 22964, ISO 6579-1, ISO 6887

agency

APHA, FDA-BAM, ISO 21527-1

agency

APHA, FDA-BAM, ISO 21527-2:2017

suitability

aerobic bacteria

suitability

Cronobacter spp., Salmonella spp., enterobacteriaceae

suitability

molds, yeasts

suitability

molds, yeasts

application(s)

microbiology, pharmaceutical

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology

application(s)

food and beverages, microbiology

pH

6.8-7.2 (25 °C, 16.1 g/L in H2O, after autoclaving)

pH

7.2 (37 °C, 25 g/L in H2O, after autoclaving)

pH

5.4-5.8 (25 °C, 32.6 g/L in H2O)

pH

5.4-5.8 (25 °C in H2O)

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

GranuCult® prime

General description

GranuCult® prime Sodium chloride peptone broth (buffered) has a combination of phosphate buffer, sodium chloride, and peptone to increase the viability of sensitive microorganisms and osmotic stability with a stable pH. The low peptone content supplies essential nutrients to maintain the viability of organisms but does not allow significant growth of more fastidious microorganisms.Surface-active ingredients or inactivators of antimicrobial agents may be added to this medium according to EP, USP, and JP.

Application

GranuCult® prime Sodium chloride peptone broth (buffered) is suitable for the dissolving, suspending, and diluting of test samples from pharmaceutical and other materials and of test strains.

Features and Benefits

  • GranuCult® offers superior granulated culture media.
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions.
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): slightly yellowish to yellowish
pH-value (25 °C): 6.8 - 7.2
Typical composition (g/litre): Potassium dihydrogen phosphate 3.6; di-Sodium hydrogen phosphate dihydrate 7.2; Sodium chloride 4.3; Peptone from meat 1.0.
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery (Escherichia coli ATCC 8739 (WDCM 00012)): 70 - 130 %
Recovery (Escherichia coli ATCC 25922 (WDCM 00013)): 70 - 130 %
Recovery (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 70 - 130 %
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): 70 - 130 %
Incubation: 45 min. at roomtemperature aerobic.
Reference medium: Tryptic Soy Agar (24 hours at 35 - 37 °C aerobic).
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

Other Notes

  • Do not use clumped or discolored medium.
  • The prepared medium is clear and slightly yellowish to yellowish

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Related product

Product No.
Description
Pricing

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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