MilliporeSigma
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77187

Millipore

Peptone Water, phosphate-buffered

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
BPW, Buffered Peptone Water, Tryptone Phosphate Water
NACRES:
NA.85

Agency

according to GB 4789.30-2016
according to GB 4789.40-2016
according to ISO 21528-1:2017
according to ISO 22964:2017

Quality Level

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

disodium hydrogen phosphate, 3.5 g/L
peptone, 10 g/L
potassium dihydrogen phosphate, 1.5 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

environmental
food and beverages
pathogen testing
sample preparation

microbiology

suitability

Providencia spp.
nonselective for Citrobacter spp.
nonselective for Cronobacter spp.
nonselective for Escherichia coli
nonselective for Klebsiella spp.
nonselective for Proteus spp.
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for Yersinia spp.
nonselective for bacteria (General Media)
nonselective for coliforms
nonselective for enterobacteriaceae

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This Item
7017977185A0715
Peptone Water suitable for microbiology, NutriSelect® Plus

Millipore

70179

Peptone Water

Millipore

Millipore

77185

Peptone Water

Andrade peptone water suitable for microbiology, NutriSelect® Plus

Millipore

A0715

Andrade peptone water

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

disodium hydrogen phosphate, 3.5 g/L , peptone, 10 g/L , potassium dihydrogen phosphate, 1.5 g/L , sodium chloride, 5 g/L

composition

peptone, 10 g/L , sodium chloride, 5 g/L

composition

casein peptone (pancreatic), 10 g/L , sodium chloride, 60 g/L , yeast extract, 3 g/L

composition

Andrade Indicator, 0.10 g/L , Peptic Digest of Animal Tissue, 10 g/L , Sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity. It is also useful for the non-selective pre-enrichment of media for bacteria, in particular pathogenic members of the Enterobacteriaceae (sub-lethal damaged), from food and other material.

Application

Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

Preparation Note

Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Studies with salmonellae

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Dario De Medici et al.
Applied and environmental microbiology, 69(6), 3456-3461 (2003-06-06)
The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis
Dongyou Liu
Molecular Detection of Foodborne Pathogens , 169-169 (2009)
Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts.
Mayachiew, Pornpimon, and Sakamon Devahastin.
LWT--Food Science and Technology, 41, 1153-1159 (2008)
H J Beckers et al.
The Journal of applied bacteriology, 62(2), 97-104 (1987-02-01)
Studies have been carried out in which growth patterns of a Salmonella sp. and competing micro-organisms, especially other Enterobacteriaceae, were followed during pre-enrichment in buffered peptone water (BPw) and subsequent selective enrichment in tetrathionate broth (TBB). Pre-enrichment cultures were inoculated

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