1.15940

Crystal violet (C.I. 42555)

for microscopy Certistain^®

• Synonym(s): Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
• Empirical Formula (Hill Notation): C₂₅H₃₀ClN₃
• CAS Number: 548-62-9
• Molecular Weight: 407.98
• MDL number: MFCD00011750
• UNSPSC Code: 41116130
• EC Index Number: 208-953-6

Properties

• Quality Level: 400
• form: solid
• potency: 420 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥88% spectrophotometric assay
• pH: 2.5-3.5 (20 °C, 10 g/L in H₂O)
• mp: 189-194 °C
• solubility: 10 g/L
• density: 1.19 g/cm³ at 20 °C
• bulk density: 220‑400 kg/m³
• εmax: 2050-2450 at 589-594 nm in water at 0.002 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

General description

Crystal violet (C.I. 42555) for microscopy Certistain^®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain^® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS07,GHS08,GHS09
• signalword: Danger
• hcodes: H302 - H318 - H351 - H410
• pcodes: P201 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3