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Millipore

Fractogel® EMD TMAE Hicap (M)

MiniChrom Column Fractogel® TMAE Hicap (M), volume 1 mL, prepacked column

Synonym(s):

MiniChrom Column Fractogel® TMAE Hicap (M), 1 ml

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About This Item

UNSPSC Code:
41115711

ligand

(Trimethylammoniumethyl)

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~180 cm/hr max. flow rate

column I.D. × L

8 mm × 20 mm

volume

1 mL

matrix active group

Methacrylate

mean particle size

40-90 μm

capacity

~180 mg, BSA binding capacity

application(s)

mAb
recombinant protein
vaccine development

separation technique

anion exchange

storage temp.

15-25°C

General description

8 x 20 mm

Features and Benefits

MiniChrom Column Fractogel® TMAE Hicap (M), 1 ml enables users to:

  • Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
  • Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Analysis Note

Appearance: passes test

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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