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1.32450

Sigma-Aldrich

AFB staining kit for histology

for the detection of acid-fast bacteria in histological tissue

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grade

histological grade

Quality Level

form

liquid

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

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AFB staining kit for histology for the detection of acid-fast bacteria in histological tissue

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Quality Level

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Quality Level

400

Quality Level

400

Quality Level

400

grade

histological grade

grade

-

grade

-

grade

-

form

liquid

form

-

form

-

form

-

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

General description

The AFB staining kit for histology - for the detection of acid-fast bacteria in histological tissue, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a ready-to-use staining kit that when used together with other in vitro diagnostic products from our portfolio makes bacterial target structures evaluable for diagnostic purposes in histological specimen materials, for example, the lung. The AFB staining kit is based on the Ziehl-Neelsen staining method for the detection of acid-fast bacteria. The set is modified such, that heating of the carbolfuchsin solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided. The use of light green solution renders the background colour less dominant, making the acid-fast bacteria considerably more visible and thus enhancing the quality of the diagnosis. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. The AFB staining kit for histology offers a modified carbol fuchsin solution which abolishes heating (Kinyoun method). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
The package is sufficient for approx. 230 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

Application

For the detection of acid-fast bacteria in histological tissue.

Analysis Note

Acid-fast bacteria: red
Background: green

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3


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