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14-392

Millipore

Green Fluorescent Protein

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Synonym(s):
GFP
eCl@ss:
32160405
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

assay

>70%

form

liquid

mol wt

Mw ~28 kDa

packaging

pkg of 300 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

GenBank® accession no.

UniProt accession no.

shipped in

dry ice

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06-896AB16901SAB4301138
vibrant-m

14-392

Green Fluorescent Protein

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: 1:1000-1:10000 (Cell Lysate)

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

UniProt accession no.

P42212

UniProt accession no.

P42212

UniProt accession no.

P42212

UniProt accession no.

-

mol wt

Mw ~28 kDa

mol wt

-

mol wt

-

mol wt

27 kDa

General description

N-terminal HIS-tagged fusion protein corresponding to full-length Green Fluorescent Protein, expressed in E. coli. Green fluorescent protein (GFP) is a cytoplasmic protein found in jellyfish Aequorea Victoria.

Biochem/physiol Actions

Green fluorescent protein (GFP) is genetically fused to many proteins in various species to generate non-reactive chimeras which are known to preserve their original biological activity as well as the fluorescent properties of native GFP. GFP acts as a reporter for gene expression and as a fusion tag for protein localization studies in live cells. It serves as a spectroscopic and microscopic probe to understand the biological functions of various systems. GFP is highly stable and can serve as a tool for in vitro studies.

Quality

Routinely evaluated as a standard on protein gels and western immunoblots, for calibration of fluorometers, and microinjection into cells and tissues.

Physical form

PBS containing 20% glycerol.

Storage and Stability

1 year at -20°C

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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