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1.50459

Supelco

SeQuant® ZIC®-pHILIC 5µm polymer 50 x 2.1 mm

PEEK-lined stainless steel column, suitable for HPLC

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material

PEEK-lined stainless steel column

Quality Level

agency

suitable for USP L122

product line

SeQuant®

feature

endcapped: no

parameter

200 bar pressure
50 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

5 cm × 2.1 mm

matrix

polymeric particle platform

matrix active group

Zwitterion bonding phase, ZIC-pHILIC

particle size

5 μm

pore size

100 Å pore size

operating pH

1-12

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

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1.504631.504601.50462
separation technique

hydrophilic interaction (HILIC)

separation technique

hydrophilic interaction (HILIC)

separation technique

hydrophilic interaction (HILIC)

separation technique

hydrophilic interaction (HILIC)

matrix

polymeric particle platform

matrix

polymeric particle platform

matrix

polymeric particle platform

matrix

polymeric particle platform

matrix active group

Zwitterion bonding phase, ZIC-pHILIC

matrix active group

Zwitterion bonding phase, ZIC-pHILIC

matrix active group

Zwitterion bonding phase, ZIC-pHILIC

matrix active group

Zwitterion bonding phase, ZIC-pHILIC

technique(s)

HPLC: suitable, UHPLC-MS: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

product line

SeQuant®

product line

SeQuant®

product line

SeQuant®

product line

SeQuant®

General description

SeQuant® ZIC®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion functional group as the silica based ZIC®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.
The column dimension of 50 mm length x 2.1 mm ID is the optimal choice for the fast separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
SeQuant® ZIC®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds.
Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant® ZIC®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD).
SeQuant® ZIC®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant® ZIC®-HILIC columns with identical functional groups, and SeQuant® ZIC®-cHILIC columns with complementary selectivity.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 40000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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