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1.51913

Supelco

Purospher® STAR NH2 (5 µm)

L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column

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Synonym(s):
Purospher® STAR NH2 (5µm) Hibar® RT 250-4.6

material

stainless steel column

agency

suitable for USP L8

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

extent of labeling

3.5% Carbon loading

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

25 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

NH2 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

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1.502451.502471.50248
Purospher® STAR NH2 (5 µm) L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column

1.51913

Purospher® STAR NH2 (5 µm)

Purospher® STAR NH2 (5 µm) L × I.D. 250 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

1.50245

Purospher® STAR NH2 (5 µm)

Purospher® STAR NH2 (5 µm) L × I.D. 150 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

1.50247

Purospher® STAR NH2 (5 µm)

Purospher® STAR NH2 (5 µm) L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

1.50248

Purospher® STAR NH2 (5 µm)

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

separation technique

hydrophilic interaction (HILIC), reversed phase, normal phase, supercritical fluid (SFC)

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

product line

Purospher®

product line

Purospher®

product line

Purospher®

product line

Purospher®

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

General description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART® 4-4 part number 1.50267.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150267]. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended for the separation of alcohols, carboxylic acids, fat-soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water-soluble vitamins.

Features and Benefits

  • Reproducible results from run-to-run and batch-to-batch

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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