MilliporeSigma
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58338

Supelco

SUPELCOSIL LC-NH2 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

EC Number:
eCl@ss:
32110501

Agency

suitable for USP L8

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g
170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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This Item
58338C4058338C3058988
agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

feature

endcapped

feature

endcapped

feature

endcapped

feature

endcapped

extent of labeling

3% Carbon loading

extent of labeling

3% Carbon loading

extent of labeling

3% Carbon loading

extent of labeling

3% Carbon loading

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform

matrix active group

amino phase

matrix active group

amino phase

matrix active group

amino phase

matrix active group

amino phase

General description

Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).
The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL LC-NH2-NP HPLC Columns for additional details).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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