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Supelco

SUPELCOSIL LC-NH2 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

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EC Number:
eCl@ss:
32110501

Agency

suitable for USP L8

Quality Level

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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This Item
58989C3058338C3059132
feature

endcapped

feature

endcapped

feature

endcapped

feature

endcapped

extent of labeling

3% Carbon loading

extent of labeling

3% Carbon loading

extent of labeling

3% Carbon loading

extent of labeling

3% Carbon loading

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

surface area

170 m2/g

surface area

170 m2/g

surface area

170 m2/g

surface area

170 m2/g

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform

General description

Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).
The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL LC-NH2-NP HPLC Columns for additional details).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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T1503
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25G
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1000309185

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Flávia Santana Moraes et al.
Food chemistry, 217, 346-351 (2016-09-25)
An analytical method was developed and validated for the determination of maltodextrin in raw milk, using high-performance liquid chromatography with evaporative light scattering detection. Maltodextrin content was evaluated in adulterated raw milk using a Supelcosil LC-NH2 (25cm×4.6mm) column and isocratic
Maria Veselá et al.
Die Nahrung, 46(4), 251-255 (2002-09-13)
The degradation fo steroid glycoalkaloids (SGAs) has been studied in model solutions. The number of colony forming units (CFU) was determined using a nondirect (cultivation) method during all stages of fermentation. The changes in SGAs content were observed by HPLC
A L Huidobro et al.
Journal of chromatography. A, 1119(1-2), 238-245 (2005-12-21)
Ibuprofen arginate is a rapidly absorbed salt designed to promote more rapid onset of analgesia than commercially available forms of ibuprofen. Ibuprofen and arginine have very different polarities and this becomes in a chromatographic problem, further complicated with the determination

Chromatograms

application for HPLC

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