MilliporeSigma
All Photos(1)

Documents

59132

Supelco

SUPELCOSIL LC-NH2-NP HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Sign Into View Organizational & Contract Pricing

Synonym(s):
SUPELCOSIL LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN
eCl@ss:
32110501

agency

suitable for USP L8

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

normal phase

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
58989C3058338C4058338C30
vibrant-m

59132

SUPELCOSIL LC-NH2-NP HPLC Column

vibrant-m

58989C30

SUPELCOSIL LC-NH2 HPLC Column

vibrant-m

58338C40

SUPELCOSIL LC-NH2 HPLC Column

vibrant-m

58338C30

SUPELCOSIL LC-NH2 HPLC Column

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform, fully porous particle

particle size

5 μm

particle size

3 μm

particle size

5 μm

particle size

5 μm

matrix active group

amino phase

matrix active group

amino phase

matrix active group

amino phase

matrix active group

amino phase

feature

endcapped

feature

endcapped

feature

endcapped

feature

endcapped

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

General description

An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.

These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.

Features and Benefits

• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

K K Peh et al.
International journal of pharmaceutical compounding, 4(3), 229-231 (2000-05-01)
A simple and selective high-performance liquid chromatography (HPLC) method using ultraviolet detection was developed for simultaneous determination of fusidic acid and betamethasone dipropionate in a cream formulation. A Supelcosil LC18 column was used for chromatographic separation. The mobile phase consisted
Sainath Babu et al.
Toxicology mechanisms and methods, 25(5), 410-416 (2015-05-30)
We have developed a simple, reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of bisphenol A (BPA) in thermal paper cash register receipts (CRs). The method is suitable for analysis of other types of bisphenols and it involves an
Vijay V Upreti et al.
Biomedical chromatography : BMC, 17(6), 385-390 (2003-09-19)
A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of DRF-1042, a novel orally active camptothecin (CPT) analog, in human plasma. The sample preparation was a simple deproteinization with acidified methanol yielding
E M Koves
Journal of chromatography. A, 692(1-2), 103-119 (1995-02-10)
A comprehensive approach to the analysis for many drugs in postmortem blood and biological fluids using high-performance liquid chromatography and diode array detection has been developed. To reduce the likelihood of co-eluting interference components of postmortem blood or other drugs
S Djabarouti et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 799(1), 165-172 (2003-12-09)
The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic (HPLC) assay for the determination of levofloxacin in human plasma, bronchoalveolar lavage and bone tissues. The sample extraction was based on a fully automated liquid-solid

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service