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58030

Sigma-Aldrich

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

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Synonym(s):
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT
Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
Molecular Weight:
505.70
Beilstein/REAXYS Number:
4093224
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioReagent

Quality Level

assay

≥97.0% (calc. on dry substance, NT)

mp

240 °C (dec.) (lit.)
~245 °C (dec.)

solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

application(s)

histology

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

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1 of 4

This Item
I8377I10406T0138
vibrant-m

58030

Iodonitrotetrazolium chloride

Essential+ Grade
vibrant-m

I8377

Iodonitrotetrazolium chloride

-
vibrant-m

I10406

Iodonitrotetrazolium chloride

-
vibrant-m

T0138

Tetrazolium Violet

-
solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

solubility

H2O: 4 mg/mL

solubility

-

solubility

methanol: 1 mg/mL, clear

assay

≥97.0% (calc. on dry substance, NT)

assay

≥98% (TLC)

assay

95%

assay

≥98% (TLC)

mp

240 °C (dec.) (lit.), ~245 °C (dec.)

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

247-249 °C

product line

BioReagent

product line

-

product line

-

product line

-

application(s)

histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

Application

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Electron acceptor for the colorimetric assay of various dehydrogenases

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

4.1B - Flammable solid hazardous materials

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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This study assessed anaerobic biodegradability of organic compounds from microorganism activity. Dehydrogenase activity can be a good parameter characterizing the microorganism activity. A modified method of 2-(p-iodophenyl-3-(p-nitrophenyl)-5-pheny tetrazolium chloride-dehydrogenase activity determination was proposed in anaerobic biodegradability assessment. Cubic spline curves

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