MilliporeSigma
  • Development of HPLC-ELSD method for determination of maltodextrin in raw milk.

Development of HPLC-ELSD method for determination of maltodextrin in raw milk.

Food chemistry (2016-09-25)
Flávia Santana Moraes, Marion Pereira da Costa, Vitor Luiz de Melo Silva, Rodrigo Vilela de Barros Pinto Moreira, Raphael Ferreira de Barros, Eliane Teixeira Mársico, Carlos Adam Conte-Junior, Adriana Cristina de Oliveira Silva
ABSTRACT

An analytical method was developed and validated for the determination of maltodextrin in raw milk, using high-performance liquid chromatography with evaporative light scattering detection. Maltodextrin content was evaluated in adulterated raw milk using a Supelcosil LC-NH2 (25cm×4.6mm) column and isocratic elution (68% of acetonitrile). Validation parameters exhibited adequate linearity, with relative standard deviation values between 0.74 and 2.16% (n=10) for repeatability and 0.11-19.39% (n=5) for intermediate precision. Limits of detection and quantification were 0.78 and 1.56mg.mL(-1), respectively, and recovery rates were between 91 and 93% for three levels. The application of this method shows that maltodextrin concentrations found in adulterated samples are lower than expected, which may be related to the quality of the commercial maltodextrin used. The method proposed proved to be simple and appropriate for the determination of maltodextrin in raw milk, with detection down to adulteration levels of 1%.

MATERIALS
Product Number
Brand
Product Description

Supelco
SUPELCOSIL LC-NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm
Supelco
SUPELCOSIL LC-NH2 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
SUPELCOSIL LC-NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
SUPELCOSIL LC-NH2 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 3 mm
Supelco
SUPELCOSIL LC-NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Supelco
SUPELCOSIL LC-NH2 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-NH2 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm
Supelco
SUPELCOSIL LC-NH2 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-NH2 HPLC Column, 3 μm particle size, L × I.D. 7.5 cm × 4.6 mm
Supelco
SUPELCOSIL LC-NH2-NP Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm