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164545

Sigma-Aldrich

7-Amino-4-methylcoumarin

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.

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Synonym(s):
7-Amino-4-methylcoumarin, AMC, Coumarin 120
Empirical Formula (Hill Notation):
C10H9NO2
CAS Number:
Molecular Weight:
175.18
MDL number:

Quality Level

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 10 mg/mL
acetone: 10 mg/mL
DMF: soluble

fluorescence

λex 365-380 nm
λem 430-460 nm

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3

InChI key

GLNDAGDHSLMOKX-UHFFFAOYSA-N

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This Item
A9891257370A3401
7-Amino-4-methylcoumarin Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.

164545

7-Amino-4-methylcoumarin

7-Amino-4-methylcoumarin Chromophore for substrates

A9891

7-Amino-4-methylcoumarin

7-Amino-4-methylcoumarin 99%

257370

7-Amino-4-methylcoumarin

solubility

DMSO: 10 mg/mL, DMF: soluble, acetone: 10 mg/mL

solubility

acetone: 10 mg/mL, clear, colorless to yellow

solubility

-

solubility

ethanol: 20 mg/mL, clear, colorless to light yellow

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

form

solid

form

powder

form

solid

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

General description

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.
Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Detection of proteolytic enzyme activity
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Other Notes

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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