MilliporeSigma
All Photos(1)

Documents

17-283

Sigma-Aldrich

Rac1 Activation Assay Kit

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

Sign Into View Organizational & Contract Pricing

eCl@ss:
32161000
NACRES:
NA.32

Quality Level

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable
affinity binding assay: suitable

UniProt accession no.

shipped in

dry ice

Gene Information

human ... RAC1(5879)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
17-44117-21805-389-AF488
vibrant-m

17-283

Rac1 Activation Assay Kit

vibrant-m

17-441

Rac1/Cdc42 Activation Assay Kit

vibrant-m

17-218

Ras Activation Assay Kit

species reactivity

human, rat, mouse

species reactivity

human, mouse, rat

species reactivity

-

species reactivity

rat, mouse, human

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

NCBI accession no.

NM_006908.3

NCBI accession no.

NM_006908.3, NM_018890.2, NM_198829.1

NCBI accession no.

NM_001085049.1, NM_012219.3

NCBI accession no.

NM_006908

UniProt accession no.

P63000

UniProt accession no.

P63000

UniProt accession no.

O14807

UniProt accession no.

P63000

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

General description

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades. The RAC1 gene is located on the human chromosome at 7p22.1.

Application

Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.

Components

Anti-Rac1, clone 23A8 (Cat.# 05-389)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Quality

Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.

Linkage

Replaces: SGT425

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Michelle E Marchese et al.
Biochemistry, 51(41), 8235-8246 (2012-09-14)
Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for
Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar et al.
Blood null
Anti-arrhythmic surgery for atrioventricular junctional and atrioventricular re-entrant tachycardia--a report of six cases.
Koo, C C, et al.
Annals of the Academy of Medicine, Singapore, 19, 67-72 (1990)
The survival kinase Mirk/dyrk1B is activated through Rac1-MKK3 signaling.
Kideok Jin, Seunghwan Lim, Stephen E Mercer, Eileen Friedman
The Journal of Biological Chemistry null
rs12977 of RAC1 is associated with the progress of gastric cancer in Iranian population
Javani A, et al.
Gene Reports, 23, 101069-101069 (2021)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service