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Sigma-Aldrich

Cdc42 Activation Assay Kit

Non-radioactive cdc42 Activation Assay Kit can be used to precipitate cdc42-GTP from cell lysates subjected to various treatments & detection by a cdc42 specific monoclonal antibody.

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eCl@ss:
32161000
NACRES:
NA.84

Quality Level

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable
affinity binding assay: suitable

UniProt accession no.

detection method

chemiluminescent

shipped in

wet ice

Gene Information

human ... CDC42(998)

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Cdc42 Activation Assay Kit

vibrant-m

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Ral Activation Assay Kit

vibrant-m

17-321

Rap1 Activation Assay Kit

vibrant-m

17-441

Rac1/Cdc42 Activation Assay Kit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Gene Information

human ... CDC42(998)

Gene Information

human ... RALA(5898)

Gene Information

human ... RAP1GDS1(5910)

Gene Information

human ... RAC1(5879)

detection method

chemiluminescent

detection method

-

detection method

-

detection method

-

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

UniProt accession no.

P60953

UniProt accession no.

P11233, P11234

UniProt accession no.

P52306

UniProt accession no.

P63000

Packaging

Kit capacity: 30 assays

Components

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Anti-cdc42

Quality

Routinely evaluated by immunoprecipitating cdc42-GTP from 3T3/NIH cell lysate that has been loaded in vitro with GTPγS. Subsequent detection in western immunoblot using anti-cdc42.

Linkage

Replaces: SGT430

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

CorrosionEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.
Zhi-Jie Cheng, Raman Deep Singh, Deepak K Sharma, Eileen L Holicky, Kentaro Hanada et al.
Molecular Biology of the Cell null
Isao Usui et al.
The Journal of biological chemistry, 278(16), 13765-13774 (2003-02-05)
We investigated the role of cdc42, a Rho GTPase family member, in insulin-induced glucose transport in 3T3-L1 adipocytes. Microinjection of anti-cdc42 antibody or cdc42 siRNA led to decreased insulin-induced and constitutively active G(q) (CA-G(q); Q209L)-induced GLUT4 translocation. Adenovirus-mediated expression of
Mark C Wilkes et al.
Developmental cell, 16(3), 433-444 (2009-03-18)
Transforming growth factor beta (TGF-beta) family ligands are pleotropic proteins with diverse cell-type-specific effects on growth and differentiation. For example, PAK2 activation is critical for the proliferative/profibrotic action of TGF-beta on mesenchymal cells, and yet it is not responsive to
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
S J Taylor et al.
Current biology : CB, 6(12), 1621-1627 (1996-12-01)
Ras proteins play an essential role in the transduction of signals from a wide range of cell-surface receptors to the nucleus. These signals may promote cellular proliferation or differentiation, depending on the cell background. It is well established that Ras

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