208920

Calmodulin Kinase IINtide

A potent and specific inhibitor of CaMK II (IC₅₀ = 50 nM for both the total and Ca²⁺-independent CaMK II activity).

• Synonym(s): Calmodulin Kinase IINtide, KRPPKLGQIGRAKRVVIEDDRIDDVLK-OH
• Empirical Formula (Hill Notation): C₁₃₆H₂₄₀N₄₄O₃₉
• Molecular Weight: 3115.63
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: off-white
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A potent and specific inhibitor of CaMK II (IC₅₀ = 50 nM for both the total and Ca²⁺-independent CaMK II activity). Exhibits inhibitory activity across converged species, including rat brain, goldfish brain, and Drosophila (IC₅₀ range 100-400 nM). Does not affect the activities of CaMK I, CaMK IV, CaMKK, PKA, or PKC. The peptide sequence corresponds to the inhibitory domain of CaMK IIN.

A potent and specific inhibitor of calmodulin kinase II (CaM-KII) (IC₅₀ = 50 nM for both total and Ca²⁺-independent activities of CaM-KII). Does not affect the activities of CaM-KI, CaM-KIV, CaM-KK, PKA, or PKC. The peptide sequence corresponds to the inhibitory domain of the inhibitory protein, CaM-KIIN. A cell-permeable version is available under Cat. No. 208921.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Cam Kinase-2

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50 nM for both the total and Ca2+-independent CaMK II activity

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Lys-Arg-Pro-Pro-Lys-Leu-Gly-Gln-Ile-Gly-Arg-Ala-Lys-Arg-Val-Val-Ile-Glu-Asp-Asp-Arg-Ile-Asp-Asp-Val-Leu-Lys-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.

Other Notes

Soderling, T.R., et al. 2001. J. Biol. Chem.276, 3719.
Chang, B.H., et al. 1998. Proc. Natl. Acad. Sci. USA95, 10890.
Pereda, A.E., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13272.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable