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G3272

Sigma-Aldrich

Guanidine hydrochloride

for molecular biology, ≥99%

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Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

biological source

synthetic (organic)

grade

for molecular biology

Assay

≥99%

form

crystalline powder

storage condition

(Tightly closed. Dry. )

technique(s)

RNA extraction: suitable

impurities

≤0.3% water (Karl Fischer)

color

white

pH

(25 °C, 4.6 - 6/573 g/L)

mp

180-185 °C (lit.)

solubility

H2O: 6 M, clear, colorless

density

1.3 g/cm3 (lit.)

cation traces

Pb: ≤5 ppm

UV absorption

λ: 260 nm Amax: ≤0.10
λ: 290 nm Amax: ≤0.05

foreign activity

DNase, RNase, none detected

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

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This Item
G45055093350950
Guanidine hydrochloride for molecular biology, ≥99%

Sigma-Aldrich

G3272

Guanidine hydrochloride

-
Guanidine hydrochloride ≥99% (titration), organic base and chaeotropic agent

Sigma-Aldrich

G4505

Guanidine hydrochloride

-
Guanidine hydrochloride BioUltra, for molecular biology, ≥99.5% (AT)

Sigma-Aldrich

50933

Guanidine hydrochloride

Premium Grade
Guanidine hydrochloride ≥98%

Sigma-Aldrich

50950

Guanidine hydrochloride

-
assay

≥99%

assay

≥99% (titration)

assay

≥99.5% (AT)

assay

≥98%

form

crystalline powder

form

crystalline powder

form

powder or crystals

form

powder or crystals

storage condition

(Tightly closed. Dry. )

storage condition

-

storage condition

-

storage condition

(Tightly closed. Dry. )

technique(s)

RNA extraction: suitable

technique(s)

RNA extraction: suitable

technique(s)

RNA extraction: suitable

technique(s)

-

impurities

≤0.3% water (Karl Fischer)

impurities

≤0.3% water (Karl Fischer)

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

-

General description

Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations.

At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.

Application

Guanidine hydrochloride has been used:
  • as a component of the extraction buffer for the extraction of proteoglycans
  • in extraction during protein fractionation of ATDC5 cell lines
  • as a chemical additive to study its effective absorbance spectra in structural analysis
  • to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
  • as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
  • in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Features and Benefits

  • Highly versatile surfactant for your cell biology and biochemical research
  • Suitable for sensitive molecular biology applications
  • Free from Exonuclease, Dnase and Rnase
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

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