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SRE0066

Sigma-Aldrich

Guanidine hydrochloride solution, 6M

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Synonym(s):
Aminoformamidine hydrochloride, GuHCl, Guanidinium chloride, Guanidium chloride
UNSPSC Code:
12352209
NACRES:
NA.25

form

liquid

Quality Level

500

concentration

6 M

application(s)

diagnostic assay manufacturing

storage temp.

room temp

Related Categories

Application

Guanidine hydrochloride is commonly used as a denaturant, because of its ability to break hydrogen bonds between amino acid residues. By breaking these bonds, the 3D conformation of the protein is unfolded and the aqueous solubility of the protein is greatly increased. Once denatured, the protein can be easily reduced, modified, or analyzed, in a variety of procedures. Further processing of the sample can allow the proteins to be analyzed by other common protein analysis methods including mass spectrometry, electrophoresis, and enzymatic digests.
Guanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.

Physical form

This product is a ready-to-use 6 M guanidine hydrochloride solution. The 6 M Guanidine hydrochloride solution may be used as a stock solution to prepare solutions of lower concentrations as appropriate.

Note: If precipitation is present in the 6 M buffer, warm the bottle to 37 °C and mix until completely dissolved prior to use.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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