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216254

Sigma-Aldrich

BCECF/AM

A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.

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Synonym(s):
BCECF/AM, 2ʹ,7ʹ- bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester, 2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
MDL number:

Quality Level

Assay

≥85% (mixture of isomers, HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white to yellow

solubility

acetonitrile: 25 mg/mL
DMF: soluble
DMSO: soluble

fluorescence

λex 500-504 nm
λem 525-531 nm

shipped in

ambient

storage temp.

−20°C

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This Item
1964193449055.09704
Sigma-Aldrich

Sigma-Aldrich

216254

BCECF/AM

BAPTA/AM Membrane-permeable form of BAPTA.

Millipore

196419

BAPTA/AM

FURA 2/AM Cell-permeable ester form of FURA 2.

Sigma-Aldrich

344905

FURA 2/AM

form

solid

form

solid

form

solid

form

powder

solubility

acetonitrile: 25 mg/mL, DMF: soluble, DMSO: soluble

solubility

DMSO: 10 mg/mL

solubility

-

solubility

DMSO: 100 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

General description

A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Monitor intracellular pH changes
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.

Other Notes

Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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