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Granzyme B Substrate II, Fluorogenic

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity.

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Synonym(s):
Granzyme B Substrate II, Fluorogenic, Z-IETD-AFC, Caspase-8 Substrate II
Empirical Formula (Hill Notation):
C37H42F3N5O13
Molecular Weight:
821.75

Quality Level

assay

≥97% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white

solubility

DMSO: 1 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

storage temp.

−20°C

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Sigma-Aldrich

218739

Caspase-1 Substrate X, Fluorogenic

form

lyophilized solid

form

lyophilized

form

solid

form

solid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, protect from light

General description

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
A fluorogenic substrate for the detection of granzyme B. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Biochem/physiol Actions

Primary Target
Fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Ile-Glu-Thr-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Karahashi, H., and Amano, F. 2000. Biol. Pharm. Bull. 23, 140.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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