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525320

Sigma-Aldrich

PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

liquid, clone PY20, Calbiochem®

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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

PY20, monoclonal

form

liquid

contains

0.1% Proclin as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

phosphorylation (pTyr)

Gene Information

human ... PID1(55022)

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525295361527NE1022
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

PY20, monoclonal

clone

PY20, monoclonal

clone

2D3, monoclonal

clone

SMI-31, monoclonal

isotype

IgG2b

isotype

IgG2b

isotype

IgG1

isotype

IgG1

form

liquid

form

liquid

form

liquid

form

liquid

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General description

Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Application

ELISA (1:2000)

Immunoblotting (1:500-1:2000)

Immunohistochemistry (1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, 1% BSA, pH 7.4.

Other Notes

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Andrew J Garton et al.
Cancer research, 66(2), 1015-1024 (2006-01-21)
OSI-930 is a novel inhibitor of the receptor tyrosine kinases Kit and kinase insert domain receptor (KDR), which is currently being evaluated in clinical studies. OSI-930 selectively inhibits Kit and KDR with similar potency in intact cells and also inhibits
Justin M Scheer et al.
PloS one, 7(12), e51817-e51817 (2013-01-04)
The structure of the Fab region of antibodies is critical to their function. By introducing single cysteine substitutions into various positions of the heavy and light chains of the Fab region of trastuzumab, a potent antagonist of HER2, and using

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