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PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem®

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biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

Drosophila, chicken, Caenorhabditis elegans, human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

phosphorylation (pThr183/pTyr185)

Gene Information

human ... MAPK8(5599)

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
  • Antibody Target Gene Symbol: MAPK8, MAPK9
  • Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
  • Entrez Gene Name: mitogen-activated protein kinase 8
  • Hu Entrez ID: 5601
  • Mu Entrez ID: 26420
  • Rat Entrez ID: 50658
  • This PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

    Immunogen

    Human
    a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK

    Application

    Immunoblotting (1:1000)

    Immunohistochemistry (see comments)

    Warning

    Toxicity: Standard Handling (A)

    Physical form

    In Dulbecco′s PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.

    Reconstitution

    Following initial thaw, aliquot and freeze (-70°C).

    Other Notes

    Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.
    Gabai, V.L., et al. 1998. FEBS Lett.438, 1.
    Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Storage Class

    12 - Non Combustible Liquids

    wgk_germany

    WGK 1

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable


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